Complement components on human lymphocytes

Lymphocytes isolated from normal human EDTA-blood were tested for the presence of cell membrane-bound complement components by reaction with specific antibodies coupled to trypsin-treated ox erythrocytes. The highest proportions of positively reacting cells were found to have C4 and β1H-globulin, wh...

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Bibliographic Details
Published inClinical immunology and immunopathology Vol. 22; no. 1; pp. 118 - 127
Main Authors Wilson, Anne B., Prichard-Thomas, Sarah, Gurner, B.W., Lachmann, P.J., Coombs, R.R.A.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 1982
Subjects
Animals
B-Lymphocytes - immunology
Cattle
Complement Activating Enzymes - immunology
Complement C1q
Complement C3 - immunology
Complement C3b Inactivator Proteins - immunology
Complement C4 - immunology
Complement C6 - immunology
Complement Factor H
Complement System Proteins - immunology
Humans
Lymphocytes - classification
Lymphocytes - immunology
Receptors, Complement
Rosette Formation
Sheep
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Summary:Lymphocytes isolated from normal human EDTA-blood were tested for the presence of cell membrane-bound complement components by reaction with specific antibodies coupled to trypsin-treated ox erythrocytes. The highest proportions of positively reacting cells were found to have C4 and β1H-globulin, which were present on 11 and 9% (mean values) of lymphocytes, respectively. Other complement components, C1q, C3, and C6, were detected on approximately 3% lymphocytes. C4 was also demonstrated on tonsil and lymph node lymphocytes. All of the cells carrying complement were C3-receptor-bearing, Ig-positive, B lymphocytes, a proportion of which also had receptors for IgG-Fc.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0090-1229
1090-2341
DOI:10.1016/0090-1229(82)90028-9