The influence of 2-mercaptoethanol (2-ME) treatment of IgG antibody on its ability to induce cytotoxicity in nonsensitized lymphocytes

The effect of 2-ME treatment of IgG on its ability to induce cytotoxicity in non-committed lymphocytes was studied. An IgG fraction of a rabbit anti-BPO serum was tested with BPO-coated chicken erythrocytes as target cells and human peripheral blood lymphocytes as effector cells. 2-Mercaptoethanol a...

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Published inCellular immunology Vol. 13; no. 3; pp. 489 - 492
Main Authors Denk, H., Stemberger, H., Wiedermann, G., Eckerstorfer, R., Tappeiner, G.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.09.1974
Subjects
Absorption
Animals
Antibodies
Antigens
Cattle - immunology
Chickens - immunology
Chromatography, Ion Exchange
Chromium Radioisotopes
Cytotoxicity Tests, Immunologic
Erythrocytes - immunology
gamma-Globulins
Humans
Immune Sera
Immunity, Cellular - drug effects
Immunoglobulin G
Lymphocytes - immunology
Mercaptoethanol - pharmacology
Penicillin G - immunology
Rabbits - immunology
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Summary:The effect of 2-ME treatment of IgG on its ability to induce cytotoxicity in non-committed lymphocytes was studied. An IgG fraction of a rabbit anti-BPO serum was tested with BPO-coated chicken erythrocytes as target cells and human peripheral blood lymphocytes as effector cells. 2-Mercaptoethanol alters the properties of the Fc fragment of the IgG antibody molecule by cleaving inter-H-chain-disulfide bonds. Treatment of the antibodies with 2-ME resulted in a significant reduction of their capacity to induce cytotoxicity of lymphocytes against the respective antigen-antibody complex. 2-Mercaptoethanol treatment may prevent conformational changes of the Fc part of the antibody molecule essential for its interaction with the Fc receptor on the effector lymphocyte.
ISSN:0008-8749
1090-2163
DOI:10.1016/0008-8749(74)90268-8