Platelet 5-hydroxytryptamine increases with platelet age in dogs

• Published in: Thrombosis and haemostasis Vol. 66; no. 2; p. 254
• Main Authors: Mezzano, D, del Pino, G E, Montesinos, M, García, M E, Aranda, E, Foradori, A
• Format: Journal Article
• Language: English
• Published: Germany 01.08.1991
• Subjects: Animals; Antibodies - administration & dosage; Blood Platelets - cytology; Blood Platelets - immunology; Blood Platelets - metabolism; Cell Survival; Disease Models, Animal; Dogs; Male; Serotonin - blood; Thrombocytopenia - blood; Thrombocytopenia - etiology; Thrombocytopenia - immunology
• ISSN: 0340-6245
• DOI: 10.1055/s-0038-1646399

Thrombocytopenia was induced in mongrel dogs by two mechanisms: immunologically, by intravenous injection of heterologous antiplatelet antibody, and non-immunologically, by circulating the blood through glass beads in anesthetized animals. The platelet content of 5-HT was monitored before and during the recovery of the blood platelet counts. This period is associated with the normalization of the mean platelet survival time and with a progressive increase in the mean age of the circulating platelet population. A continuous increment in platelet 5-HT closely followed the increase in platelet counts in both models of thrombocytopenia, and a strong correlation was found between the platelet age and 5-HT content. These findings support the concept that platelets accumulate 5-HT during their physiological aging process, contradicting the notion that a negative balance in 5-HT content results at the end of their physiological lifespan in circulation. These results are not in conflict with the concept that circulating platelets release and re-uptake 5-HT.