Fluorometric determination of tocopherol in sheep plasma

A simple, sensitive and precise method is described for the fluorometric determination of tocopherol in sheep plasma. Precautions taken at each step of the procedure to guard against oxidative losses give recoveries of 93–100%. The variation in free tocopherol level with seasonal pasture change and...

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Bibliographic Details
Published inBiochemical medicine Vol. 11; no. 1; pp. 71 - 80
Main Author Storer, G.B.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.09.1974
Subjects
Animal Feed
Animals
Ascorbic Acid
Chromatography, Thin Layer
Cyclohexanes
Diet
Evaluation Studies as Topic
Female
Hydrogen-Ion Concentration
Indicators and Reagents
Methods
Microchemistry
Pregnancy
Seasons
Sheep - blood
Spectrometry, Fluorescence
Time Factors
Vitamin E - blood
Weaning
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Summary:A simple, sensitive and precise method is described for the fluorometric determination of tocopherol in sheep plasma. Precautions taken at each step of the procedure to guard against oxidative losses give recoveries of 93–100%. The variation in free tocopherol level with seasonal pasture change and pregnancy ranged from 128 μg tocopherol 100 ml plasma (SE 6.76) in nonpregnant ewes on dry summer feed to 284 μg tocopherol 100 ml (SE 10.4) at full term.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0006-2944
1557-7996
DOI:10.1016/0006-2944(74)90097-0