Changes in the pattern of phospholipid synthesis during the induction by cytokinin of cell division in soybean suspension cultures

A method is described for preparing fully viable, cytokinin-starved soybean (Glycine max (L.) Merr. cv. Acme) cells from a suspension-culture of callus tissue. The cells respond to kinetin treatment by re-initiating cell division. We present evidence, from the pattern of incorporation of (32)P-label...

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Bibliographic Details
Published inPlanta Vol. 170; no. 2; p. 161
Main Authors Connett, R.J.A. (Botany School, Cambridge (UK)), Hanke, D.E
Format Journal Article
LanguageEnglish
Published Germany 01.02.1987
Subjects
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
CELL CULTURE
CITOCININAS
CULTIVO DE CELULAS
CULTURE DE CELLULES
Cytokinin
CYTOKININE
CYTOKININS
FOSFOLIPIDOS
Glycine
GLYCINE MAX
MITOSE
MITOSIS
PHOSPHATIDE
Phospholipid
PHOSPHOLIPIDS
Phosphorisotop
Suspensionskultur
Synthese
Wachstumsregulator
Zellteilung
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Summary:A method is described for preparing fully viable, cytokinin-starved soybean (Glycine max (L.) Merr. cv. Acme) cells from a suspension-culture of callus tissue. The cells respond to kinetin treatment by re-initiating cell division. We present evidence, from the pattern of incorporation of (32)P-labelled inorganic phosphate into individual phospholipids during the first hour of this response, that the synthesis of phosphatidylinositol (PI) and of phosphatidic-acid head-groups is affected within 15 min. The polyphosphoinositide phosphatidylinositol 4-phosphate, but not phosphatidylinositol 4,5-bisphosphate, was detected in the tissue. The characteristics of cytokinin-induced PI synthesis in cytokinin-starved soybean cells appear to resemble the 'PI response' of animal cells.
Bibliography:87G022088
F60
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00397884