The repair of radiation injury in L-cells treated by adriamycin

• Published in: European journal of cancer Vol. 16; no. 3; pp. 333 - 338
• Main Authors: Bistrović, M., Nagy, B., Maričić, Ž.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.1980
• Subjects: Cell Survival - drug effects; Cell Survival - radiation effects; Dose-Response Relationship, Radiation; Doxorubicin - pharmacology; Kinetics; L Cells (Cell Line) - drug effects; L Cells (Cell Line) - radiation effects; Time Factors
• ISSN: 0014-2964
• DOI: 10.1016/0014-2964(80)90349-7

The effect of adriamycin on the radiation survival curve before, during and after treatment was studied in L-cells. The radiation survival curve became exponential when the cells treated with sufficient concentration of adriamycin, were irradiated at a specific time. The ‘time’ factor therefore must also be considered an essential parameter for interaction. The time dependence (kinetics) of survival was determined by treating the cells with 0.5 μ g/ml ( 0.1 μ g/ml) adriamycin for 4 hr (6 hr) and irradiating them at various intervals with 600 rad (700 rad). The most intensive interaction was found to be 3 hr after addition of the drug. No reparatory survival increase was observed in the double dose experiment immediately following addition of the drug. This is not due to the actual absence of repair, but is rather caused by the growing intensity of the adriamycin-radiation interaction. When the interaction was maximal and stationary during the double dose experiment, no suppression of early repair was observed.