Effect of calcium on cyclic nucleotide phosphodiesterase in parathyroid tissue

• Published in: Biochemical and biophysical research communications Vol. 94; no. 3; pp. 763 - 768
• Main Authors: Willgoss, D., Jacobi, J.M., de Jersey, J., Bartley, P.C., Lloyd, H.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.06.1980
• Subjects: 3',5'-Cyclic-AMP Phosphodiesterases - isolation & purification; 3',5'-Cyclic-AMP Phosphodiesterases - metabolism; 3',5'-Cyclic-GMP Phosphodiesterases - isolation & purification; 3',5'-Cyclic-GMP Phosphodiesterases - metabolism; Animals; Calcium - pharmacology; Cattle; Egtazic Acid - pharmacology; Parathyroid Glands - drug effects; Parathyroid Glands - enzymology; cyclic AMP; cyclic GMP; EGTA
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(80)91300-5

The hydrolysis of cyclic AMP and cyclic GMP by homogenates of normal bovine parathyroid gland and human parathyroid adenomas was decreased by EGTA. When supernatants were chromatographed on DEAE-cellulose it was found that sheep brain calmodulin in the presence of calcium stimulated cyclic AMP and cyclic GMP phosphodiesterase activity. The response to calmodulin in two human parathyroid adenomas was less than that in normal bovine parathyroid. Calmodulin was detected in heat-treated supernatants of 11 parathyroid adenomas by its ability to activate calmodulin-free sheep brain phosphodiesterase. The results suggest a role for calcium in the hydrolysis of cyclic nucleotides in parathyroid tissue.