The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat

• Published in: Euphytica Vol. 113; no. 3; pp. 163 - 185
• Main Authors: Gupta, P.K., Varshney, R.K.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.01.2000; Springer Nature B.V
• Subjects: agricultural programs and projects; Agronomy. Soil science and plant productions; Biological and medical sciences; chromosome mapping; Classical and quantitative genetics. Population genetics. Molecular genetics; cytogenetics; DNA fingerprinting; Fundamental and applied biological sciences. Psychology; Gene mapping; Generalities. Genetics. Plant material; genetic markers; Genetics; Genetics and breeding of economic plants; germplasm; microsatellite repeats; Molecular genetics; nucleic acid hybridization; Plant breeding; polymerase chain reaction; Triticum aestivum; Wheat; Genetic mapping; Molecular hybridization; Monocotyledones; Genome organization; Genetic marker; Genetic resource; Genetic diversity; Review; Gene library; Molecular marker; Cereal crop; Polymerase chain reaction; Allele; Germplasm; Gramineae; Microsatellite DNA; Fingerprint method; Genetic improvement; Angiospermae; Spermatophyta; Oligonucleotide; Molecular cloning; Triticum aestivum; Polymorphism
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1023/A:1003910819967

In recent years, a variety of molecular markers, based on microsatellites or simple sequence repeats (SSRs) have become the markers of choice, thus necessitating their development and use in a variety of plant systems. In this review, the basic principles underlying different hybridization-based (oligonucleotide fingerprinting) and PCR based approaches (STMS, MP-PCR, AMP-PCR/ ISSR/ ASSR, RAMPs/ dRAMPs, SAMPL), making use of microsatellites, have been outlined. Different methods for enrichment of genomic libraries for microsatellites have also been outlined. Relevant literature on the subject, giving a summary of results obtained using each approach, has been reviewed and critically discussed. The review also includes a discussion on literature, which deals with the use of microsatellites in genome mapping, gene tagging, DNA fingerprinting, characterization of germplasm and cytogenetics research. Special emphasis has been laid on the genome of bread wheat, where the work done in the authors' own laboratory has also been briefly reviewed.[PUBLICATION ABSTRACT]