Profiling urinary steroids. A reliable procedure

A relatively simple method of gas chromatography, permiting the quantitative estimation of the most important urinary 17-ketosteroids and 17-OH corticosteroids, is reported. The procedure consists in enzyme hydrolysis, dichloromethane extraction, and (without any further purification) gas-liquid chr...

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Bibliographic Details
Published inClinica chimica acta Vol. 38; no. 1; pp. 103 - 111
Main Authors Van de Calseyde, J.F., Scholtis, R.J.H., Schmidt, N.A., Leijten, C.J.J.A.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.04.1972
Subjects
17-Hydroxycorticosteroids - urine
17-Ketosteroids - urine
Adolescent
Adult
Chemical Phenomena
Chemistry
Chromatography, Gas
Female
Glucuronidase
Humans
Hydrocarbons, Halogenated
Hydrolysis
Kinetics
Male
Mathematics
Methods
Oximes
Silicon
Snails
Solubility
BSA
O-bis
TSIM
TMCS
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Summary:A relatively simple method of gas chromatography, permiting the quantitative estimation of the most important urinary 17-ketosteroids and 17-OH corticosteroids, is reported. The procedure consists in enzyme hydrolysis, dichloromethane extraction, and (without any further purification) gas-liquid chromatography of the steroids as their o-methyloxime-trimethyl silyi ether (MO-TMS) derivatives. The method is shown to be useful in clinical studies.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(72)90214-8