Hepatic aldehyde dehydrogenases and lipid peroxidation
Recent findings have shown that microsomal membrane lipid peroxidation generates a variety of reactive aldehydic products. The interaction of lipid peroxidation products with hepatic aldehyde dehydrogenases (ALDH) was studied using rat liver subcellular fractions. The well-documented membrane peroxi...
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Published in | Pharmacology, biochemistry and behavior Vol. 18; pp. 155 - 160 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
1983
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Subjects | |
Online Access | Get full text |
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Summary: | Recent findings have shown that microsomal membrane lipid peroxidation generates a variety of reactive aldehydic products. The interaction of lipid peroxidation products with hepatic aldehyde dehydrogenases (ALDH) was studied using rat liver subcellular fractions. The well-documented membrane peroxidation product malondialdehyde (MDA) was studied to determine if ALDH isozymes play a role in metabolism of this aldehyde. The cytosolic and mitochondrial hepatic subcellular fractions were found to contain ALDH isozymes capable of oxidizing MDA. The kinetic properties of a cytosolic ALDH (K
m of approximately 16 μM) suggest that this enzyme may be involved in the metabolism of MDA
in vivo. Both the cytosolic and mitochondrial fractions also contained an ALDH isozyme with K
m values in the millimolar range. Addition of the cytosolic fraction of rat liver produced a significant decrease in the accumulation of MDA during CCl
4-induced microsomal membrane lipid peroxidation but did not protect cytochrome P-450 from destruction. The mitochandrial low K
m ALDH isozyme was found to be a target enzyme for inhibition during
in vitro microsomal lipid peroxidation. These studies show that a select ALDH isozyme is sensitive to inhibition during membrane lipid peroxidation whereas other isozymes may be involved in the metabolism of aldehydic peroxidation products. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0091-3057 1873-5177 |
DOI: | 10.1016/0091-3057(83)90164-8 |