FTIR-ATR studies of the effect of shear rate upon albumin adsorption onto polyurethaneurea
The effect of wall shear rate upon albumin adsorption onto a polyurethaneurea, Biomer® † † Biomer® is a registered trademark of Ethicon, Inc., USA. , was studied using FTIR-ATR spectroscopy. In these experiments, nondiffusion conrolled adsorption was observed from 10 mg/ml solutions at known wall sh...
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| Published in | Biomaterials Vol. 7; no. 5; pp. 340 - 347 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier Ltd
01.09.1986
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| Subjects | |
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| Abstract | The effect of wall shear rate upon albumin adsorption onto a polyurethaneurea, Biomer®
†
†
Biomer® is a registered trademark of Ethicon, Inc., USA.
, was studied using FTIR-ATR spectroscopy. In these experiments, nondiffusion conrolled adsorption was observed from 10 mg/ml solutions at known wall shear rates. Results show that increasing wall shear rate during adsorption does not significantly affect the adsorption kinetics, but does decrease the rate of protein desorption. These results suggest that albumin adsorbed at higher shear rates is more tightly bound to the surface. Comparison of the data to simple mathematical models suggests that more than one layer of protein adsorbs in 2 h. |
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| AbstractList | The effect of wall shear rate upon albumin adsorption onto a polyurethaneurea, Biomer, was studied using FTIR-ATR spectroscopy. In these experiments, nondiffusion controlled adsorption was observed from 10 mg/ml solutions at known wall shear rates. Results show that increasing wall shear rate during adsorption does not significantly affect the adsorption kinetics, but does decrease the rate of protein desorption. These results suggest that albumin adsorbed at higher shear rates is more tightly bound to the surface. Comparison of the data to simple mathematical models suggests that more than one layer of protein adsorbs in 2 h. The effect of wall shear rate upon albumin adsorption onto a polyurethaneurea, Biomer® † † Biomer® is a registered trademark of Ethicon, Inc., USA. , was studied using FTIR-ATR spectroscopy. In these experiments, nondiffusion conrolled adsorption was observed from 10 mg/ml solutions at known wall shear rates. Results show that increasing wall shear rate during adsorption does not significantly affect the adsorption kinetics, but does decrease the rate of protein desorption. These results suggest that albumin adsorbed at higher shear rates is more tightly bound to the surface. Comparison of the data to simple mathematical models suggests that more than one layer of protein adsorbs in 2 h. |
| Author | Cooper, S.L. Pitt, W.G. |
| Author_xml | – sequence: 1 givenname: W.G. surname: Pitt fullname: Pitt, W.G. – sequence: 2 givenname: S.L. surname: Cooper fullname: Cooper, S.L. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/3778993$$D View this record in MEDLINE/PubMed |
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| Keywords | protein adsorption shear rate FTIR-ATR albumin polyurethaneurea Polymers protein desorption Biomef |
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| Snippet | The effect of wall shear rate upon albumin adsorption onto a polyurethaneurea, Biomer®
†
†
Biomer® is a registered trademark of Ethicon, Inc., USA.
, was... The effect of wall shear rate upon albumin adsorption onto a polyurethaneurea, Biomer, was studied using FTIR-ATR spectroscopy. In these experiments,... |
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| SubjectTerms | Adsorption albumin Biocompatible Materials Biomef FTIR-ATR Kinetics Models, Chemical Polymers Polyurethanes polyurethaneurea protein adsorption Protein Binding protein desorption Serum Albumin, Bovine shear rate Spectrophotometry, Infrared Surface Properties |
| Title | FTIR-ATR studies of the effect of shear rate upon albumin adsorption onto polyurethaneurea |
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