Convection-free boundary sedimentation of dilute protein solutions

Use of the density gradient sedimentation velocity technique appears to be essential for the accurate determination of the mean sedimentation coefficients of dilute protein solutions. When performed on an analytical ultracentrifuge equipped with a photoelectric-scanning-absorption optical system, th...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 54; no. 2; pp. 586 - 591
Main Authors Noel, J.K.Fuller, Schumaker, V.N.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.08.1973
Subjects
Animals
Centrifugation, Density Gradient
Glyceraldehyde-3-Phosphate Dehydrogenases
Macromolecular Substances
Methods
Muscles - enzymology
Rabbits
Time Factors
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Summary:Use of the density gradient sedimentation velocity technique appears to be essential for the accurate determination of the mean sedimentation coefficients of dilute protein solutions. When performed on an analytical ultracentrifuge equipped with a photoelectric-scanning-absorption optical system, the density gradient sedimentation velocity technique has been shown to be particularly useful in studying the subunit association-dissociation equilibria of multisubunit enzyme systems. The time factor has been shown to be a major advantage of the density gradient sedimentation velocity technique, as opposed to the sedimentation equilibrium technique, in studying the subunit association-dissociation equilibria of multisubunit enzymes such as rabbit muscle apo-glyceraldehyde-3-phosphate dehydrogenase, which is very unstable in dilute solution.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(73)90390-4