Extraction and structural properties of Acanthophora muscoides (Rhodophyceae) extracellular matrix sulfated polysaccharides and their effects on coagulation

• Published in: Acta scientiarum. Technology Vol. 38; no. 3; pp. 273 - 282
• Main Authors: Rodrigues, José Ariévilo Gurgel, Queiroz, Ismael Nilo Lino de, Quinderé, Ana Luíza Gomes, Benevides, Norma Maria Barros, Tovar, Ana Maria Freire, Mourão, Paulo Antônio De Souza
• Format: Journal Article
• Language: English
• Published: Maringa Editora da Universidade Estadual de Maringá - EDUEM 2016; Universidade Estadual de Maringá
• Subjects: cell-wall; clotting; polyanionics; Rhodophyceae; structural chemistry
• ISSN: 1806-2563; 1807-8664; 1806-2563
• DOI: 10.4025/actascitechnol.v38i3.26146

Acanthophora muscoides (Rhodophyta) contains structurally heterogeneous sulfated polysaccharides (Am-SPs) with pharmacological importance; however, its matrix SPs composition has not been still extensively investigated. This study sequentially extracted and compared the structural features and the in vitro anticoagulant effects of the Am-SPs. Papain-extraction sequence yielded Am.E-1, Am.E-2 and Am.E-3 containing differences among the relative proportions of sulfate (26.18-33%) and hexoses (42.02-60.67%) based on chemical analyses. One- (1H) and two-dimensions (1H/13C) nuclear magnetic resonance experiments showed very complex Am-SPs composed of alternating 4-linked-α-galactopyranosyl units and 3-linked-β-galactopyranosyl units presenting variable sulfation, CH3 substitutions and3,6-anhydro-α-L-galactose units and pyruvated-D-galactose residues, respectively, typical of agarocolloids. Different chromatographic profiles (DEAE-cellulose) were observed, with fractions (Am I, Am II and Am III eluted with 0.5, 0.75 and/or 1 M of NaCl, respectively) revealing charge density patterns and distinct mobility to heparin by agarose-electrophoresis and, when analyzed by polyacrylamide-electrophoresis, a dispersive migration and similar mobility as chondroitin-6-sulfate for Am I fractions were noted. Regarding the activated partial thromboplastin time test, fractions had no virtually anticoagulation (1.47[arrow right]3.07 IU mg-1) in comparison with 193 IU mg-1 heparin. Therefore, Am-SPs show significantly lower anticoagulation than heparin.