Chromosomal and phylogenetic context for conglutin genes in Arachis based on genomic sequence

Comparative genomic and cDNA sequence analysis of ara h 2, a major peanut allergen, and a related conglutin ara h 6 were performed in Arachis hypogaea L. and its putative progenitors, Arachis duranensis and Arachis ipaensis. The complete identity between sequences encoding Ara h 2 isoforms demonstra...

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Published inMolecular genetics and genomics : MGG Vol. 275; no. 6; pp. 578 - 592
Main Authors Ramos, M Laura, Fleming, Geraldine, Chu, Ye, Akiyama, Yukio, Gallo, Maria, Ozias-Akins, Peggy
Format Journal Article
LanguageEnglish
Published Germany Springer Nature B.V 01.06.2006
Subjects
Arachis
Arachis - genetics
Arachis hypogaea
Base Sequence
Blotting, Southern
Chromosomes, Plant
DNA, Plant
Genes
Genetics
Genome, Plant
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Phylogeny
Plant Proteins - genetics
Sequence Homology, Nucleic Acid
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Summary:Comparative genomic and cDNA sequence analysis of ara h 2, a major peanut allergen, and a related conglutin ara h 6 were performed in Arachis hypogaea L. and its putative progenitors, Arachis duranensis and Arachis ipaensis. The complete identity between sequences encoding Ara h 2 isoforms demonstrated that these are homeologous genes and represent orthologs from diploid ancestors. Three copies of ara h 6 were identified in A. hypogaea, one of them located in the A-genome and the other two in the B-genome. Expression analysis showed higher levels of ara h 2 transcripts compared with ara h 6. Dual-labeled genomic in situ hybridization permitted identification of two subgenomes, each of which contained one pair of ara h 2-ara h 6 signals localized by fluorescence in situ hybridization. Characterization of genomic clones showed close genetic linkage between Ara h 2.02 and one copy of ara h 6 in the B-genome. The physical linkage may have arisen by tandem duplication and divergence of an ancestral gene. A gene duplication event specific to the B-genome progenitor has resulted in ara h 6 paralogs. These data provide further evidence for progenitor relationships and genomic organization of the conglutin gene family in the genus Arachis and could contribute to the development of a hypoallergenic peanut.
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ISSN:1617-4615
1617-4623
DOI:10.1007/s00438-006-0114-z