The l-arabinose permease system in Escherichia coli B/r

The l-arabinose permease system of Escherichia coli B/r was characterized by measuring l-[ i- 14C]arabinose uptake by an l-arabinose isomerase-less mutant. This organism accumulates l-arabinose via an inducible, energy-dependent system that exhibits the kinetic properties of permease-mediated transp...

Full description

Saved in:
Bibliographic Details
Published inBiochimica et biophysica acta Vol. 117; no. 1; pp. 217 - 230
Main Authors Novotny, Charles P., Englesberg, Ellis
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 28.03.1966
Subjects
Arabinose - metabolism
Bacterial Proteins - metabolism
Biological Transport
Escherichia coli - metabolism
Fucose - metabolism
Galactose - metabolism
Kinetics
Membrane Transport Proteins - metabolism
Mutation
Xylose - metabolism
αMG
TMG
Online AccessGet full text

Cover

More Information
Summary:The l-arabinose permease system of Escherichia coli B/r was characterized by measuring l-[ i- 14C]arabinose uptake by an l-arabinose isomerase-less mutant. This organism accumulates l-arabinose via an inducible, energy-dependent system that exhibits the kinetic properties of permease-mediated transport. The system consists of at least 2 parts: an entrance reaction that obeys Michealis-Menten kinetics and an exit reaction with a high temperature coefficient that exhibits first-order kinetics. Inhibition studies indicate that the system may also mediate the transport of d-xylose, d-frucose, and d-galactose. A “cold shock” phenomenon which results in a rapid loss in accumulated carbohydrates is described.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(66)90169-3