Evidence for age-dependent impairment of ovalbumin heterogeneous nuclear RNA (HnRNA) processing in hen oviduct

The expression of the ovalbumin gene in hen oviduct decreases during ageing. Northern transfer of electrophoretically fractionated total RNA from oviducts of mature egg-laying and old non-egg-laying hens, and hybridization to nick-translated plasmid pOV230 (ovalbumin complementary DNA cloned into pM...

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Bibliographic Details
Published inMechanisms of ageing and development Vol. 30; no. 3; pp. 319 - 324
Main Authors Schröder, Heinz C., Messer, Rudolf, Breter, Hans-Joachim, Müller, Werner E.G.
Format Journal Article
LanguageEnglish
Published Ireland Elsevier Ireland Ltd 31.05.1985
Subjects
Age Factors
Aging
Animals
Chickens
Female
Hen oviduct
HnRNA
Northern transfer
Ovalbumin - analysis
Ovalbumin - genetics
Ovalbumin gene
Oviducts - metabolism
Processing
RNA Processing, Post-Transcriptional
RNA, Heterogeneous Nuclear - metabolism
Hen oviduct
Ovalbumin gene
Northern transfer
HnRNA
Processing
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Summary:The expression of the ovalbumin gene in hen oviduct decreases during ageing. Northern transfer of electrophoretically fractionated total RNA from oviducts of mature egg-laying and old non-egg-laying hens, and hybridization to nick-translated plasmid pOV230 (ovalbumin complementary DNA cloned into pMB9) revealed with age increasing concentrations of distinct high-molecular-weight ovalbumin pre-mRNA species as compared with that of functional 18S ovalbumin mRNA. The accumulation of ovalbumin sequence-containing processing intermediates in old hen oviducts indicates lowered rates of RNA splicing and/or altered steady state concentrations of splicing intermediates during post-transcriptional maturation of heterogeneous nuclear RNA in old animals.
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ISSN:0047-6374
1872-6216
DOI:10.1016/0047-6374(85)90120-4