Expression of globin genes in teratocarcinoma-Friend cell hybrids

We have used an isoelectric focusing technique to analyse the hemoglobins synthesized by cell hybrids isolated following the fusion of Friend erythroleukemia to embryonal carcinoma cells. Our results confirm that the embryonal carcinoma-derived alpha globin genes are expressed in cell hybrids. In ad...

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Bibliographic Details
Published inExperimental cell research Vol. 131; no. 2; pp. 277 - 282
Main Authors McBurney, Michael, Craig, Jane, Stedman, Deborah, Featherstone, Mark
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.02.1981
Subjects
Animals
Dimethyl Sulfoxide - pharmacology
Friend murine leukemia virus
Gene Expression Regulation
Globins - genetics
Hemin - pharmacology
Hybrid Cells - physiology
Isoelectric Point
Leukemia, Erythroblastic, Acute - genetics
Mice
Teratoma - genetics
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Summary:We have used an isoelectric focusing technique to analyse the hemoglobins synthesized by cell hybrids isolated following the fusion of Friend erythroleukemia to embryonal carcinoma cells. Our results confirm that the embryonal carcinoma-derived alpha globin genes are expressed in cell hybrids. In addition, the extent to which the various alpha chains and beta chains are synthesized in these hybrids depends on both the genetic composition of the cell line and on the chemical nature of the agent used to induce hemoglobin synthesis.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(81)90232-9