ELISA for in vivo assessment of nonenzymatically glycated platelet glutathione peroxidase

• Published in: Clinical biochemistry Vol. 27; no. 4; pp. 293 - 298
• Main Authors: Muraganandam, Arumugam, Tannous, Marie, Mutus, Bulent
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.1994
• Subjects: Animals; Antibody Specificity; Blood Platelets - enzymology; Blotting, Western; Boronic Acids - chemistry; Cattle; Chromatography, Affinity; Computer Simulation; diabetes; Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 1 - enzymology; Electrophoresis, Polyacrylamide Gel; ELISA; Enzyme-Linked Immunosorbent Assay; Erythrocytes - enzymology; glutathione peroxidase; Glutathione Peroxidase - blood; Glycosylation; Humans; Immune Sera - immunology; Linear Models; m-aminophenylboronate; nonenzymatic glycation; platelet; platelet; nonenzymatic glycation; diabetes; m-aminophenylboronate; glutathione peroxidase; ELISA
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/0009-9120(94)00023-9

Using a combination of boronate affinity chromatography and ELISA methodology, a simple procedure was devised to selectively determine the in vivo glycated state of the platelet glutathione peroxidase (GSH-Px) from normal and diabetic subjects. The mean total GSH-Px levels in the normal ( n = 14) and diabetic ( n = 18) platelets were 1167 ± 97 and 1007 ± 73 ng/mg protein, respectively. The mean percentage glycated GSH-Px in the normal and diabetic platelets were 5.79 ± 0.72% and 11.68 ± 0.95%, respectively. When the percentage glycated GSH-Px was compared with the fructosamine values, a correlation coefficient of 0.71 was obtained. This indicates that the glycation status of platelet GSH-Px can be utilized as a sensitive, short-term index of plasma glucose levels.