Liquid scintillation counting of radioactive monomeric and polymeric carbohydrates on paper chromatograms

A simple, improved scintillation counting procedure was developed for the assay of radioactive mono- and polysaccharides on paper chromatograms. Segments of chromatograms are placed in scintillation vials and soaked in water to completely elute the carbohydrate before addition of Aquasol, a xylene-b...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 56; no. 2; pp. 443 - 449
Main Authors Sandford, Paul A., Watson, Paul R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.1973
Subjects
Carbohydrates - analysis
Carbon Radioisotopes
Chromatography, Paper
Glucosamine - analysis
Manometry
Methods
Methylglycosides - analysis
Molecular Weight
Polysaccharides - analysis
Scintillation Counting
Solvents
Tritium
Yeasts
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Summary:A simple, improved scintillation counting procedure was developed for the assay of radioactive mono- and polysaccharides on paper chromatograms. Segments of chromatograms are placed in scintillation vials and soaked in water to completely elute the carbohydrate before addition of Aquasol, a xylene-based scintillation fluid. The resulting water-Aquasol solution is counted in a liquid scintillation counter. Evaluation of numerous experimental variables revealed optimal conditions for complete elution of mono- and polysaccharides with water before counting in Aquasol. The water elution-Aquasol procedure allows water-soluble substances ( 14C- and 3H-labeled) on paper to be assayed with increased accuracy and sensitivity (three- to fivefold improvement in counting efficiency of tritiated samples). The simplicity of the procedure allows entire radiochromatograms to be assayed readily.
Bibliography:ObjectType-Article-1
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(73)90211-X