A semi-quantitative micromethod for the determination of free glycosaminoglycans in serum. Results from studies on serum of healthy children of various age and patients affected by mucopolysaccharidosis
A method is presented for the determination of free glycosaminoglycan (GAG) concentration in as little as 30 μl serum. By filtration of the serum through DEAE-cellulose paper, the free GAG fraction is selectively adsorbed and concentrated on a circular area of 15.9 mm 2; these GAG spots are stained...
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Published in | Clinica chimica acta Vol. 108; no. 2; pp. 179 - 188 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
08.12.1980
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Subjects | |
Online Access | Get full text |
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Summary: | A method is presented for the determination of free glycosaminoglycan (GAG) concentration in as little as 30 μl serum. By filtration of the serum through DEAE-cellulose paper, the free GAG fraction is selectively adsorbed and concentrated on a circular area of 15.9 mm
2; these GAG spots are stained with Alcian Blue. The relationship between the amounts of adsorbed GAG and the optical density of the Alcian Blue spots is linear with a certain range; e.g. for chondroitin sulfate (mixed isomers), from 0.25 to 1.00 μg. With this method — which we will refer to as the “DEAE Alcian Blue” method — we estimated the free GAG concentration in sera of individuals of various ages including newborns. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0009-8981 1873-3492 |
DOI: | 10.1016/0009-8981(80)90003-0 |