A semi-quantitative micromethod for the determination of free glycosaminoglycans in serum. Results from studies on serum of healthy children of various age and patients affected by mucopolysaccharidosis

A method is presented for the determination of free glycosaminoglycan (GAG) concentration in as little as 30 μl serum. By filtration of the serum through DEAE-cellulose paper, the free GAG fraction is selectively adsorbed and concentrated on a circular area of 15.9 mm 2; these GAG spots are stained...

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Published inClinica chimica acta Vol. 108; no. 2; pp. 179 - 188
Main Authors Melet, J., Hooghwinkel, G.J.M., Giesberts, M.A.H., Van Gelderen, H.H.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 08.12.1980
Subjects
Adolescent
Adult
Aging
Alcian Blue
Child
Child, Preschool
Female
Glycosaminoglycans - blood
Humans
Infant
Infant, Newborn
Infant, Premature
Male
Microchemistry
Middle Aged
Mucopolysaccharidoses - blood
Mucopolysaccharidosis I - blood
Mucopolysaccharidosis III - blood
Spectrophotometry - methods
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Summary:A method is presented for the determination of free glycosaminoglycan (GAG) concentration in as little as 30 μl serum. By filtration of the serum through DEAE-cellulose paper, the free GAG fraction is selectively adsorbed and concentrated on a circular area of 15.9 mm 2; these GAG spots are stained with Alcian Blue. The relationship between the amounts of adsorbed GAG and the optical density of the Alcian Blue spots is linear with a certain range; e.g. for chondroitin sulfate (mixed isomers), from 0.25 to 1.00 μg. With this method — which we will refer to as the “DEAE Alcian Blue” method — we estimated the free GAG concentration in sera of individuals of various ages including newborns.
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ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(80)90003-0