Separation of cellular phospholipid, neutral lipid and cholesterol by high-pressure liquid chromatography

High-pressure liquid chromatography was used to separate cellular cholesterol, cholesteryl esters, free fatty acids, triacylglycerols and phospholipids. Application of the technique toward quantitative analysis of cellular sterol, phospholipid and neutral lipid content and metabolism is described.

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 665; no. 3; pp. 355 - 358
Main Authors Schlager, Seymour I., Jordi, Howard
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 24.09.1981
Subjects
Animals
Cells - analysis
Cholesterol
Cholesterol - isolation & purification
Cholesterol Esters - isolation & purification
Chromatography, High Pressure Liquid - methods
Fatty Acids, Nonesterified - isolation & purification
High-pressure liquid chromatography
Lipids - isolation & purification
Neutral lipid
Phospholipid
Phospholipids - isolation & purification
Triglycerides - isolation & purification
Phospholipid
Cholesterol
High-pressure liquid chromatography
Neutral lipid
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Summary:High-pressure liquid chromatography was used to separate cellular cholesterol, cholesteryl esters, free fatty acids, triacylglycerols and phospholipids. Application of the technique toward quantitative analysis of cellular sterol, phospholipid and neutral lipid content and metabolism is described.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0005-2760
0006-3002
1879-145X
DOI:10.1016/0005-2760(81)90246-0