Pathology of mosquito iridescent virus of Aedes taeniorhynchus in cell cultures of Aedes aegypti

An electron microscopical study was conducted on the pathology of the mosquito iridescent virus (MIV) of Aedes taeniorhynchus in monolayer cultures of Aedes aegypti cells. The sequence of events in the pathology, from the initiation of attachment through maturation and release, is presented. MIV att...

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Bibliographic Details
Published inJournal of invertebrate pathology Vol. 27; no. 1; pp. 27 - 40
Main Authors Webb, S.R., Paschke, J.D., Wagner, G.W., Campbell, W.R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 1976
Subjects
Aedes - microbiology
Animals
Cells, Cultured
Insect Viruses - ultrastructure
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Summary:An electron microscopical study was conducted on the pathology of the mosquito iridescent virus (MIV) of Aedes taeniorhynchus in monolayer cultures of Aedes aegypti cells. The sequence of events in the pathology, from the initiation of attachment through maturation and release, is presented. MIV attaches to cells and is taken up by the process of viropexis (phagocytosis) within 15 min after inoculation. Intact virions are released into the cytoplasm at 30–60 min by disruption of the phagocytic vesicles. Discrete foci of replication (viroplasm) develop in the cytoplasm within 1 day after infection. Progeny virus is assembled in the viroplasm within 2 days after infection and later appears at the cell surface, where it acquires an envelope from the plasma membrane upon budding from the cell. Virus does not accumulate to form aggregates in the cytoplasm; instead, it buds from the cell after assembly.
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ISSN:0022-2011
1096-0805
DOI:10.1016/0022-2011(76)90025-2