Di-leucine-mediated Internalization of Ligand by a Truncated Growth Hormone Receptor Is Independent of the Ubiquitin Conjugation System
The growth hormone receptor (GHR) is a member of the cytokine receptor family. Its function is to mediate cellular responses upon binding of growth hormone. Ligand binding induces dimerization and activation of the GHR. One mechanism by which the GHR is rapidly inactivated involves the ubiquitin con...
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Published in | The Journal of biological chemistry Vol. 273; no. 26; pp. 16426 - 16433 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
26.06.1998
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Subjects | |
Online Access | Get full text |
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Summary: | The growth hormone receptor (GHR) is a member of the cytokine receptor family. Its function is to mediate cellular responses
upon binding of growth hormone. Ligand binding induces dimerization and activation of the GHR. One mechanism by which the
GHR is rapidly inactivated involves the ubiquitin conjugation system, a system implicated in the degradation of cytosolic
and nuclear proteins. We have shown previously that the ubiquitin-conjugating system mediates internalization of the GHR.
Here, we present evidence that in addition to the ubiquitin-dependent endocytosis signal, the cytosolic tail of the GHR contains
a di-leucine motif. Upon truncation of the GHR at amino acid residue 349, this di-leucine motif is activated and mediates
ubiquitin-independent internalization of the receptor. Di-leucine-mediated GHR internalization requires functional clathrin-coated
pits and results in GHR transport to the lysosome. Although the full-length GHR internalizes independent of the di-leucine
motif, this motif may function in internalization of GHR isoforms. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.273.26.16426 |