Simultaneous determination of E- and Z-guggulsterones in dietary supplements containing Commiphora mukul extract (guggulipid) by liquid chromatography

• Published in: Journal of AOAC International Vol. 84; no. 1; p. 24
• Main Authors: Nagarajan, M, Waszkuc, T W, Sun, J
• Format: Journal Article
• Language: English
• Published: England 01.01.2001
• Subjects: Chromatography, Liquid; Dietary Supplements - analysis; Hypolipidemic Agents - analysis; Hypolipidemic Agents - chemistry; India; Indicators and Reagents; Plant Extracts - analysis; Plants, Medicinal - chemistry; Pregnenediones - analysis; Pregnenediones - chemistry; Reference Standards; Reproducibility of Results; Stereoisomerism; India
• ISSN: 1060-3271
• DOI: 10.1093/jaoac/84.1.24

Guggulipid, the standardized product from the extraction of the ole-gum-resin from the Commiphora mukul plant, has been marketed as a hypolipidemic agent. The ketosteroids, cis- and trans-4,17(20)-pregnadiene-3,16-dione, known as E- and Z-guggulsterones, respectively, are the main ingredients in guggulipid. A liquid chromatographic method was developed for simultaneous determination of E- and Z-guggulsterones in guggulipid preparations using synthetic E- and Z-guggulsterone standards. Realtively low amounts of guggulsterones (E and Z) were found in commercial guggulipid preparations in comparison with the manufacturer's claim of 2.5%. The mixture of E- and Z-guggulsterones was extracted and separated on a Symmetry C18 reversed-phase column, with a mobile phase of acetonitrile--water (46 + 54, v/v) and detected at 242 nm. The retention times of E- and Z-guggulsterones are approximately 8 and 11 min, respectively. Assay quantitation was based on the calibration curve obtained from a mixture of synthetic standard E- and Z-guggulsterones. Experimental data on selectivity, linearity, accuracy, and recoveries are presented.