Polymer ultrathin films with immobilized photosynthetic reaction center proteins

• Published in: Macromolecular symposia. Vol. 106; no. 1; pp. 193 - 203
• Main Authors: Kalabina, N.A, Zaitsev, S. Yu, Zubov, V.P, Lukashev, E.P, Kononenko, A.A
• Format: Journal Article
• Language: English
• Published: Hüthig & Wepf Verlag 01.04.1996
• ISSN: 1022-1360; 1521-3900
• DOI: 10.1002/masy.19961060118

Properties of mixed monolayers of lipid-photosynthetic reaction center proteins (RC) were studied and the optimum conditions for stable films fabrication were determined. The following synthetic: N-acryloylphosphatidylethanolamine (ACPE), tetracosa-11, 13-diinoic acid (TDA), pentacosa-10, 12-diinoic acid (PDA), dioctadecyldienoylphosphatidylcholine (DODL) and natural lipids: L-α-phosphatidylethanolamine (PE), L-α-phosphatidylcholine (PC) were used. The rate of polymerization of the mixed ACPE-RC and TDA-RC monolayers is lower in comparison with corresponding values for pure lipid-like monomers on air/water interface. The optical and photoelectrical measurements provide evidence for an orientation of RCs on interface. Hydrophilic H-subunit in monomeric and polymeric ACPE-RCs, and monomeric DODL-RCs monolayers is preferentially oriented towards water as in the pure RC monolayers. Opposite orientation was found with TDA-RCs and PDA-RCs films. No preferential orientation for lipid-RCs from C. aurantiacus monolayers was found because of the RCs having low assymmetry of hydrophobic subunits (M and L).