Variations of antioxidative responses in two rice cultivars with polyamine treatment under salinity stress

• Published in: Physiology and molecular biology of plants Vol. 18; no. 4; pp. 301 - 313
• Main Authors: Ghosh, N., Das, Soumya Prakash, Mandal, C., Gupta, Sudha, Das, Kingsuk, Dey, N., Adak, M. K.
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.10.2012
• Subjects: Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Cell Biology; Life Sciences; Plant Physiology; Plant Sciences; Research Article; Lipid peroxidation; Polyamine; Antioxidative enzymes; Rice; Salinity
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-012-0124-8

The rice varieties viz . Nonabokra and Swarna were evaluated on the basis of their responses for oxidative stress induced by sodium chloride (NaCl) and the effects of exogenously applied polyamine thereon. Rice seedlings were treated with 200 mM of NaCl supplemented with two dosages: 1 mM and 2 mM putrescine. Following treatments, plants were evaluated for accumulation of reactive oxygen species (ROS) like O 2 − , H 2 O 2 etc. in tissues, lipid peroxidation, protein carbonylation, accumulation of flavonoids and anthocyanin, activities of different oxidative enzymes like guaiacol peroxidase (GPX), catalase (CAT) and glutathione reductase (GR). Preliminary, oxidative stress out of salinity was ensured by plants from significantly higher accumulation of O 2 − and H 2 O 2 in the tissues of the NaCl treated varieties. Irrespective of varieties, there recorded a significant variation of the endogenous polyamine profiles under NaCl stress. Interestingly, exogenous application of putrescine had a close relationship on O 2 − and H 2 O 2 content for both the varieties. However, Nonabokra was evident as more respondent than Swarna to applied putrescine. The other effects of oxidative stress was impacted on plants as higher values of MDA content, enhanced rate of protein oxidation and putrescine recorded as an alleviating agent regardless of varieties with dose dependant manner. The generation of ROS and cellular disintegration was accompanied by up regulation of non-enzymatic and enzymatic antioxidation pathways with exogenous application of putrescine. For non-enzymatic antioxidant, it revealed that putrescine was highly effective for sustaining the anthocyanin and flavonoid content in both the varieties under salinity. Whereas, antioxidative enzyme, CAT showed its diminished activity; but activity of GPX and GR were significantly induced under salinity and it was according to the concentration of applied putrescine.