Structured phosphatidylcholine with elevated content of conjugated linoleic acid: Optimization by response surface methodology

• Published in: European journal of lipid science and technology Vol. 114; no. 11; pp. 1261 - 1267
• Main Authors: Baeza-Jiménez, Ramiro, Noriega-Rodríguez, Juan A., García, Hugo S., Otero, Cristina
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.11.2012; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Biological and medical sciences; Conjugated linoleic acid; Fat industries; Food industries; Fundamental and applied biological sciences. Psychology; Phosphatidylcholine; Phospholipase A1; Response surface methodology; Structured phospholipids; Conjugated linoleic acid; Structured phospholipids; Methodology; Enzyme; Phospholipid; Lipids; Esterases; Response surface methodology; Phospholipase A; Optimization; Carboxylic ester hydrolases; Oils and fats industry; Phosphatidylcholine; Hydrolases; Response surface
• ISSN: 1438-7697; 1438-9312
• DOI: 10.1002/ejlt.201200038

Structured phosphatidylcholine was successfully produced by immobilized phospholipase A1 catalyzed acidolysis of phosphatidylcholine and CLA. Response surface methodology was applied to optimize the reaction system using three process parameters: enzyme load, temperature, and substrates molar ratio. Optimal conditions obtained from the model were 15% enzyme load at 55°C for a 1:4 (PC/CLA) substrates molar ratio, which produced a yield of 85.8% of CLA incorporation. The total correlation coefficient (R2) was 0.92 and no lack of fit was detected. This suggests the fitness of the model obtained and it suggests that the model is sufficiently accurate to estimate the incorporation of CLA into PC. Practical applications: This study could contribute to process development for enzyme catalyzed phospholipid modification. By changing the fatty acid composition of lecithin, delivery of desired (beneficial) fatty acids could be better achieved. Additionally, emulsification properties of the phospholipids could open a wide area of applications in the food and cosmetic industries. A plot representing the reaction scheme for the production of structured phosphatidylcholine (SPC). The mechanisms for SPC formation in the acidolysis reactions studied also involve hydrolysis and esterification reactions. As a result, lysophosphatidylcholine (LPC) is produced in the first step and it quickly becomes a reactant in the second step. The amount of LPC in the reaction mixture affects the overall reaction rate and also causes acyl migration or by‐products formation, and as a consequence the formation of LPC decreases the yield and purity of SPC.