Biocompatibility of mesoporous SBA‐16/hydroxyapatite nanocomposite and dentin demineralized particles on human dental pulp stem cells

• Published in: Microscopy research and technique Vol. 85; no. 4; pp. 1557 - 1567
• Main Authors: Bulajić, Dragica V., Drljača, Jovana, Čapo, Ivan, Savić, Slavica M., Vojisavljević, Katarina, Hodžić, Aden, Sekulić, Slobodan, Bajkin, Branislav V.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.04.2022; Wiley Subscription Services, Inc
• Subjects: Aged; Biocompatibility; Biomaterials; Biomedical materials; Bio‐Oss; Bone growth; Cell viability; Cytotoxicity; Dental materials; Dental Pulp; Dentin; Dentin - chemistry; Durapatite - chemistry; Flow cytometry; Fourier transforms; Humans; Hydroxyapatite; Infrared spectroscopy; Microenvironments; Nanocomposites; Phenotypes; Regeneration; Regeneration (physiology); SBA‐16/HA; Scanning electron microscopy; SHED; Sheds; Silica; Silicon dioxide; Silicon Dioxide - analysis; Silicon Dioxide - chemistry; Silicon Dioxide - pharmacology; Spectrum analysis; Stem Cells; Substrates; Teeth; Toxicity; X-Ray Diffraction; SBA-16/HA; SHED; Bio-Oss; dentin; biocompatibility; cytotoxicity
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.24017

In the present work, a biomaterial (SBA‐16/HA) based on the growth of hydroxyapatite (HA) particles within an organized silica structure SBA‐16 (Santa Barbara Amorphous‐16) was developed to evaluate its application to act as a porous microenvironment promoting attachment and viability of human dental pulp stem cells of healthy deciduous teeth (SHED). First, SHED were isolated and their phenotypes were evaluated by flow cytometry. The samples of SBA‐16/HA were characterized by X‐ray diffraction (XRD), small and wide angle X‐ray scattering (SWAXS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) equipped with energy dispersive spectra detector (EDS). Afterward, cells were cultured in the eluates of the above‐mentioned biomaterial aged for 24 hr, 7. and 14 days. Bio‐Oss® and dentin particles are involved for comparison and cells are cultured in the eluates of these two materials also. Thiazolyl Blue Tetrazolium bromide assay‐MTT (3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide assay) was used for the determination of cell viability. The results obtained by all aforementioned characterization methods of SBA‐16/HA, revealed a uniform spherical mesoporous structure, an intrinsic characteristic of this material. This material displayed excellent biocompatibility on SHEDs, and even proliferative potential, indicating that SBA‐16/HA could potentially serve as a suitable substrate for bone regeneration. Contrary to SBA‐16/HA, dentin particles showed low cytotoxicity at all time points, compared to control and Bio‐Oss®groups. Our results substantiate the idea that SBA‐16/HA has a beneficial effect on SHEDs, thus paving the way toward developing new material for bone replacement. Dental pulp stem cells had excellent viability in contact with SBA‐16/HA scaffold. Dentin particles were rated slightly cytotoxic for dental pulp stem cells. SBA‐16/HA can be highlighted as a promising strategy for orofacial bone reconstruction.