Enhanced US/CT/MR imaging of integrin αvβ3 for liver fibrosis staging in rat
Liver fibrosis is a global health challenge with high morbidity and mortality rates, and diagnostic sensitivity of liver fibrosis tests can be increased using multimodal molecular agents. We designed cyclic arginine-glycine-aspartic acid (cRGD)-modified nanoparticles (NPs) using ultrasound (US)/comp...
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Published in | Frontiers in chemistry Vol. 10; p. 996116 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
03.10.2022
|
Subjects | |
Online Access | Get full text |
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Summary: | Liver fibrosis is a global health challenge with high morbidity and mortality rates, and diagnostic sensitivity of liver fibrosis tests can be increased using multimodal molecular agents. We designed cyclic arginine-glycine-aspartic acid (cRGD)-modified nanoparticles (NPs) using ultrasound (US)/computed tomography (CT)/magnetic resonance (MR) triple-modality imaging to evaluate liver fibrosis stages.
In vitro
and
in vivo
studies were conducted using primary hepatic stellate cells (HSCs) and a rat model of liver fibrosis induced by carbon tetrachloride (CCl
4
). Our results showed cRGD-poly(lactic-co-glycolic acid)-Fe
3
O
4
-perfluorocarbon bromide (cRGD-PLGA-Fe
3
O
4
-PFOB) NPs were preferentially internalised by activated HSCs (aHSCs). The main cell types expressing integrin α
v
β
3
during liver fibrogenesis were the aHSCs. The protein levels of α
v
and β
3
expressed on aHSCs increased with the progression of liver fibrosis. After intravenous injection of cRGD-PLGA-Fe
3
O
4
-PFOB NPs, the echo intensity (EI) values, CT values, and T2 values of liver parenchyma correlated well with liver fibrosis severity. cRGD-PLGA-Fe
3
O
4
-PFOB NPs as multifunction contrast agents showed great potential to reflect the degree of HSC activation and distinguish among different liver fibrotic stages. The ligand-directed and integrin α
v
β
3
-mediated accumulation provides active and passive targeting capabilities, permitting the targeted multimodal imaging of cRGD-PLGA-Fe
3
O
4
-PFOB NPs, which delivers accurate non-invasive diagnosis and real-time monitoring of liver fibrosis development. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Xinhui Su, Zhejiang University, China Edited by: Yizhou Dong, The Ohio State University, United States Reviewed by: Hongyu Lin, Xiamen University, China These authors have contributed equally to this work This article was submitted to Medicinal and Pharmaceutical Chemistry, a section of the journal Frontiers in Chemistry |
ISSN: | 2296-2646 2296-2646 |
DOI: | 10.3389/fchem.2022.996116 |