Inorganic pyrophosphatase from the red flour beetle (Tribolium castaneum) modulates mitochondrial polyphosphate metabolism

Polyphosphates (polyPs) have been found in all cell types examined to date and play diverse roles, depending on the cell type. In eukaryotic organisms, polyPs have been mainly investigated in mammalian cells, with few studies on insects. In this study, we investigated mitochondrial polyphosphate met...

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Published inArchives of insect biochemistry and physiology Vol. 102; no. 4; pp. e21606 - n/a
Main Authors Mirra, Bianca, Carvalho, Klébea, Curitiba, Bianca, Ribeiro, Lupis, Moraes, Jorge, da Silva, José R., Costa, Evenilton P., da Fonseca, Rodrigo N., Campos, Eldo
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.12.2019
Subjects
Adenosine triphosphatase
Adenosine Triphosphatases
Animals
Beetles
Coleoptera
Double-stranded RNA
Exopolyphosphatase
Female
Flour
Inorganic pyrophosphatase
Inorganic Pyrophosphatase - chemistry
Inorganic Pyrophosphatase - metabolism
insect
Insect Proteins - metabolism
Insects
Isoforms
Male
Mammalian cells
Metabolism
Mitochondria
Mitochondria - metabolism
polyphosphate
Polyphosphates
Polyphosphates - metabolism
Pyrophosphatase
RNA Interference
RNA-mediated interference
Substrate specificity
Substrates
Tribolium - enzymology
Tribolium - metabolism
Tribolium castaneum
Tripolyphosphate
polyphosphate
insect
metabolism
mitochondria
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Summary:Polyphosphates (polyPs) have been found in all cell types examined to date and play diverse roles, depending on the cell type. In eukaryotic organisms, polyPs have been mainly investigated in mammalian cells, with few studies on insects. In this study, we investigated mitochondrial polyphosphate metabolism in the red flour beetle, Tribolium castaneum. Substrate specificity for different chain lengths demonstrated the presence of two exopolyphosphatase isoforms in mitochondria. T. castaneum mitochondrial polyP levels decreased after injection with soluble pyrophosphatase (Tc‐sPPase) dsRNA, while the membrane exopolyphosphate activity increased. Mitochondrial respiration modulated exopolyphosphatase activity only in wild‐type beetles. Tripolyphosphate was able to increase the F‐ATPase activity in wild‐type and Tc‐sPPase RNAi beetles. We suggest that inorganic pyrophosphatase modulates polyphosphate metabolism in mitochondria and affects the link between mitochondrial activity and polyphosphate metabolism in T. castaneum. The inorganic pyrophosphatase affects polyphosphate and mitochondrial metabolism
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ISSN:0739-4462
1520-6327
DOI:10.1002/arch.21606