Light and transmission electron microscopic structure of skin glands and dermal scales of a caecilian amphibian Gegeneophis ramaswamii, with a note on antimicrobial property of skin gland secretion

• Published in: Microscopy research and technique Vol. 82; no. 8; pp. 1267 - 1276
• Main Authors: Arun, Damodaran, Akbarsha, Mohammad A., Oommen, Oommen V., Divya, Lekha
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.08.2019; Wiley Subscription Services, Inc
• Subjects: Amphibians; Antibacterial activity; antimicrobial; Antimicrobial agents; Bioactive compounds; caecilian; cutaneous glands; dermal scales; E coli; Electron microscopy; G. ramaswamii; Gegeneophis ramaswamii; Glands; Gram-negative bacteria; Granular materials; Homeostasis; Klebsiella; Light microscopy; Microscopy; Predators; Reptiles & amphibians; Scanning electron microscopy; Secretions; Skin; antimicrobial; G. ramaswamii; dermal scales; cutaneous glands; caecilian
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.23276

Amphibian skin secretions contain a variety of bioactive compounds that are involved in diverse roles such as communication, homeostasis, defence against predators, pathogens, and so on. Especially, the caecilian amphibians possess numerous cutaneous glands that produce the secretory material, which facilitate survival in their harsh subterranean environment. Inspite of the fact that India has a fairly abundant distribution of caecilian amphibians, there has hardly been any study on their skin and its secretion. Herein, we describe, using light microscopy and electron microscopy, two types of dermal glands, mucous and granular, in Gegeneophis ramaswamii. The mucous glands are filled with mucous materials. The mucous‐producing cells are located near the periphery. The granular glands are surrounded by myoepithelial cells. A large number of granules of different sizes are present in the lumen of the granular gland. The granule‐producing cells are present near the myoepithelial lining of the gland. There are small flat disk‐like dermal scales in pockets in the transverse ridges of the posterior region of the body. Each pocket contains 1–4 scales of various sizes. Scanning electron microscopic (SEM) study of the skin surface showed numerous funnel‐shaped glandular openings. The antibacterial activity of the skin secretions was revealed in the test against Escherichia coli, Klebsiella pneumoniae, and Aeromonas hydrophila, all gram‐negative bacteria. SEM analyses confirm the membrane damage in bacterial cells on exposure to skin secretions of G. ramaswamii. 1. Light and electron microscopic study of the skin of G. ramaswamii. 2. Mucous and granular glands are reported. 3. Dermal scales are found in the posterior body region. 4. First report on gegeneophis species. 5. Antimicrobial property of the skin secretion was explored.