Applications of yeast flocculation in biotechnological processes

A review on the main aspects associated with yeast flocculation and its application in biotechnological processes is presented. This subject is addressed following three main aspects--the basics of yeast flocculation, the development of "new" flocculating yeast strains and bioreactor devel...

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Published inBiotechnology and bioprocess engineering Vol. 5; no. 4; pp. 288 - 305
Main Authors Domingues, Lucília, Vicente, António A., Lima, Nelson, Teixeira, José A.
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.08.2000
Subjects
beta -Galactosidase
Bioreactors
Biotechnology
Brewing yeast
Cell culture
Cell density
Ethanol
Fermentation
Flocculation
Galactosidase
Genetics
Hydrodynamics
Lactose
Mass transfer
Microbiology
Reviews
Saccharomyces cerevisiae
Solid phases
Yeast
Yeasts
β-Galactosidase
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ISSN1226-8372
1976-3816
DOI10.1007/BF02942185

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Summary:A review on the main aspects associated with yeast flocculation and its application in biotechnological processes is presented. This subject is addressed following three main aspects--the basics of yeast flocculation, the development of "new" flocculating yeast strains and bioreactor development. In what concerns the basics of yeast flocculation, the state of the art on the most relevant aspects of mechanism, physiology and genetics of yeast flocculation is reported. The construction of flocculating yeast strains includes not only the recombinant constitutive flocculent brewer's yeast, but also recombinant flocculent yeast for lactose metabolisation and ethanol production. Furthermore, recent work on the heterologous β-galactosidase production using a recombinant flocculentSaccharomyces cerevisiae is considered. As bioreactors using flocculating yeast cells have particular properties, mainly associated with a high solid phase hold-up, a section dedicated to its operation is presented. Aspects such as bioreactor productivity and culture stability as well as bioreactor hydrodynamics and mass transfer properties of flocculating cell cultures are considered. Finally, the paper concludes describing some of the applications of high cell density flocculation bioreactors and discussing potential new uses of these systems.[PUBLICATION ABSTRACT]
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ISSN:1226-8372
1976-3816
DOI:10.1007/BF02942185