The importance of protoporphyrin IX efflux for ALA-PDT dosimetry

One of the major advances in PDT is the use of 5‐aminolevulinic acid (ALA) to induce the production of an endogenous photosensitizer inside the cells using intracellular enzymatic pathways. ALA is the first intermediate in heme biosynthesis and a precursor of the protoporphyrin IX (PpIX). When activ...

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Bibliographic Details
Published inLaser physics letters Vol. 6; no. 8; pp. 611 - 615
Main Authors Milanetto, M.C., Imasato, H., Perussi, J.R.
Format Journal Article
LanguageEnglish
Published Berlin WILEY-VCH Verlag 01.08.2009
WILEY‐VCH Verlag
Wiley
Subjects
5-aminolevulinic acid
Biological and medical applications
Biological and medical sciences
cytotoxicity
Exact sciences and technology
Fundamental areas of phenomenology (including applications)
Medical sciences
Optics
photodynamic therapy
photosensitizer
Physics
protoporphyrin IX
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Technology. Biomaterials. Equipments. Material. Instrumentation
Aluminium arsenides
87.50.-a
87.64.-t
Transmission electron microscopy
Photodynamic therapy
87.19.Xx
protoporphyrin IX
Dosimetry
Laser applications in medicine
5-aminolevulinic acid
cytotoxicity
photosensitizer
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Summary:One of the major advances in PDT is the use of 5‐aminolevulinic acid (ALA) to induce the production of an endogenous photosensitizer inside the cells using intracellular enzymatic pathways. ALA is the first intermediate in heme biosynthesis and a precursor of the protoporphyrin IX (PpIX). When activated by light, this efficient photosensitizer accumulated in the target cells can produce cytotoxicity. The aim of this study was to find the best conditions for cell killing using ALA to temporarily increase the concentration of PpIX in two cell lines. It was shown that a considerable efflux of synthesized PpIX occurs. Since this efflux is time‐dependent, it is essential to know the optimum time for irradiation after ALA administration. So, the efflux of PpIX from the cells is an important parameter to be considered for ALA‐PDT dosimetry. (© 2009 by Astro Ltd., Published exclusively by WILEY‐VCH Verlag GmbH & Co. KGaA)
Bibliography:MEC-SESu
FAPESP
ark:/67375/WNG-V30FBSM6-R
CNPq
ArticleID:LAPL200910045
istex:68ACF9B9770CED6DABBA36836DFF1E4531C8E0DF
ISSN:1612-2011
1612-202X
DOI:10.1002/lapl.200910045