MiR-324-5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8

• Published in: Journal of pharmacy and pharmacology Vol. 70; no. 11; p. 1513
• Main Authors: Lin, Hai, Zhou, Ai-Jun, Zhang, Jing-Yu, Liu, Shu-Fang, Gu, Jian-Xiang
• Format: Journal Article
• Language: English
• Published: England 01.11.2018
• Subjects: Animals; Apoptosis - drug effects; Cell Line, Tumor; Female; Gene Expression Regulation, Neoplastic; Humans; Mice, Inbred BALB C; Mice, Nude; MicroRNAs - genetics; MicroRNAs - metabolism; Signal Transduction; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Tetraspanins - genetics; Tetraspanins - metabolism; Tumor Burden; apoptosis; MiR-324-5p; TSPAN8; gastric cancer; viability
• ISSN: 2042-7158
• DOI: 10.1111/jphp.12995

The purpose of this study was to further clarify the role and underlying mechanism of miR-324-5p in gastric cancer. The expressions of miR-324-5p and TSPAN8 as determined by qRT-PCR or Western blot were compared between the gastric cancer tissues and normal tissues. Human gastric cancer cell line SGC-7901 was cultured and transfected with miR-324-5p mimic/inhibitor or pcDNA-TSPAN8. The cell survival was assessed by the cell viability and apoptosis. Luciferase reporter gene assays were performed to explore the interaction between miR-324-5p and TSPAN8 in SGC-7901 cells. MiR-324-5p was decreased in human gastric carcinoma tissues (n = 33), but TSPAN8 protein expression was increased in the gastric carcinoma tissues (n = 33). Moreover, miR-324-5p inhibited the viability and induced the apoptosis of gastric cancer cells in vitro. TSPAN8 is a functional target of miR-324-5p in gastric cancer. MiR-324-5p was further confirmed to reduce gastric cancer cell viability and induce apoptosis via downregulating TSPAN8 in SGC-7901 cells in vitro. Additionally, miR-324-5p overexpression markedly inhibited the tumorigenesis of gastric cancer cells in vivo, as shown by the smaller tumour volume compared with the control. This study suggested a novel, probable mechanism of miR-324-5p in gastric cancer context and revealed that miR-324-5p inhibited gastric cancer cell survival by targeting TSPAN8.