Multidrug resistance phenotype: Relation between phenotype induction and its characteristics in erythroleukemia cells

• Published in: Cell biology international Vol. 43; no. 2; pp. 214 - 219
• Main Authors: Soares, Fernanda Saldanha, Lettnin, Aline Portantiolo, Wagner, Eduardo Felipe, Mattozo, Francielly Hafele, Carrett‐Dias, Michele, Rumjanek, Vivian Mary Barral Dodd, Filgueira, Daza Moraes Vaz Batista, de Souza Votto, Ana Paula
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.02.2019
• Subjects: Apoptosis; Apoptosis - drug effects; Apoptosis - radiation effects; Cancer; Cell Line, Tumor; Cell membranes; Cell Survival - drug effects; Cell Survival - radiation effects; Chemotherapy; Cytotoxicity; Deoxyribonucleic acid; DNA; Drug Resistance, Neoplasm; Erythroleukemia; Genotype & phenotype; H2O2; Humans; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; K562 Cells; leukemia; Leukemia, Erythroblastic, Acute - metabolism; Leukemia, Erythroblastic, Acute - pathology; MDR phenotype; Multidrug resistance; Multidrug resistant organisms; Phenotype; Phenotypes; Reactive Oxygen Species - metabolism; Ultraviolet radiation; Ultraviolet Rays; UVA; UVB; UVB; UVA; leukemia; MDR phenotype; H2O2
• ISSN: 1065-6995; 1095-8355
• DOI: 10.1002/cbin.11095

Chemotherapy may be followed by multiple drug resistance (MDR). This is an obstacle in the treatment of cancer. It is therefore essential to understand the mechanisms underlying tumor resistance, especially those involved in the cell target/MDR relationship. To investigate this, the effects of exposing cells to UVB (to target DNA), UVA, and H2O2 (to target the cell membrane) were observed in K562 (non MDR) and FEPS (MDR) cell lines. The K562 cells were more sensitive to UVA than the FEPS cells. The FEPS cell line was more resistant to H2O2 than K562, only presenting cytotoxicity 72 h after being exposed to 40 mM, with no ROS increase until 48 h. Both cell lines were sensitive to UVB, presenting cytotoxicity after 24 h, mainly by apoptosis, and showed an increase in ROS levels. Our results indicate that agents acting on DNA may be able to overcome the MDR phenotype.