Interleukin 1 enhances growth factor-dependent proliferation of the clonogenic cells in acute myeloblastic leukemia and of normal human primitive hemopoietic precursors

• Published in: The Journal of experimental medicine Vol. 168; no. 2; pp. 463 - 474
• Main Authors: TRANG HOANG, HAMAN, A, GONCALVES, O, LETENDRE, F, MATHIEU, M, WONG, G. G, CLARK, S. C
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.08.1988; The Rockefeller University Press
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Biological and medical sciences; Bone Marrow Cells; Cell Division - drug effects; Cells, Cultured; Colony-Forming Units Assay; Colony-Stimulating Factors - immunology; Colony-Stimulating Factors - pharmacology; Drug Synergism; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - drug effects; Humans; Immune Sera; Immunobiology; Interleukin-1 - genetics; Interleukin-1 - physiology; Kinetics; Leukemia, Myeloid, Acute - pathology; Lymphokines, interleukins ( function, expression); Nucleic Acid Hybridization; Recombinant Proteins - pharmacology; Regulatory factors and their cellular receptors; Tumor Cells, Cultured - cytology; Tumor Cells, Cultured - drug effects; Tumor Stem Cell Assay; Cell proliferation; Stimulation; Malignant hemopathy; Interleukin 1; Growth factor; Acute myelocytic leukemia
• ISSN: 0022-1007; 1540-9538
• DOI: 10.1084/jem.168.2.463

IL-1 is released by activated monocytes and is thought to be a key mediator of the host immune response. The availability of the purified and, more recently, recombinant IL-1 has allowed the characterization of other biological properties of this molecule. Thus, IL-1 is thought to have the same properties as hemopoietic 1, a growth factor that has been shown to act on primitive murine hemopoietic cells. Here we report that rIL-1 acts synergistically with granulocyte/macrophage CSF (GM-CSF) or granulocyte CSF in the stimulation of clonogenic cells from many patients with acute myeloblastic leukemia (AML). Although IL-1 by itself has no effect on AML blasts, it can support colony formation under conditions where there is detectable production of endogenous GM-CSF. IL-1 also promotes the growth of multipotential progenitors from normal human bone marrow cells in the presence of GM-CSF. These observations support the hypothesis that in the hemopoietic system, IL-1 has a selective effect on primitive precursors.