Natural Deep Eutectic Solvents as Performance Additives for Peroxygenase Catalysis

Natural deep eutectic solvents (NADES) are proposed as alternative solvents for peroxygenase‐catalysed oxyfunctionalization reactions. Choline chloride‐based NADES are of particular interest as they can serve as solvent, enzyme‐stabiliser and sacrificial electron donor for the in situ H2O2 generatio...

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Published inChemCatChem Vol. 12; no. 4; pp. 989 - 994
Main Authors Ma, Yunjian, Li, Yongru, Ali, Shahid, Li, Peilin, Zhang, Wuyuan, Rauch, Marine C. R., Willot, Sébastien J.‐P., Ribitsch, Doris, Choi, Young Hae, Alcalde, Miguel, Hollmann, Frank, Wang, Yonghua
Format Journal Article
LanguageEnglish
Published Weinheim Wiley Subscription Services, Inc 20.02.2020
Subjects
Additives
Choline
Choline oxidase
Deep Eutectic Solvents
Hydrogen peroxide
Oxyfunctionalisation reactions
Peroxygenases
Solvents
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Summary:Natural deep eutectic solvents (NADES) are proposed as alternative solvents for peroxygenase‐catalysed oxyfunctionalization reactions. Choline chloride‐based NADES are of particular interest as they can serve as solvent, enzyme‐stabiliser and sacrificial electron donor for the in situ H2O2 generation. This report provides the first proof‐of‐concept and basic characterisation of this new reaction system. Highly promising turnover numbers for the biocatalysts of up to 200,000 have been achieved. Natural Deep Eutectic Solvents: Envisioned bienzymatic cascade for the selective oxyfunctionalisation of ethyl benzene or cis‐ß‐methylstyrene comprising the evolved peroxygenase from Agrocybe aegerita (rAaeUPO) and choline oxidase from Arthrobacter nicotianae (AnChOx) to provide rAaeUPO with H2O2.
ISSN:1867-3880
1867-3899
DOI:10.1002/cctc.201901842