pH Mapping of Gliomas Using Quantitative Chemical Exchange Saturation Transfer MRI: Quasi‐Steady‐State, Spillover‐, and MT‐Corrected Omega Plot Analysis

• Published in: Journal of magnetic resonance imaging Vol. 60; no. 4; pp. 1444 - 1455
• Main Authors: Liu, Ying, Wu, Yin, Ji, Yang, Zhao, Botao, Jin, Ziyi, Ju, Shenghong, Chu, Ying‐Hua, Liebig, Patrick Alexander, Wang, He, Li, Cong, Zhang, Xiao‐Yong
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.10.2024; Wiley Subscription Services, Inc
• Subjects: Animal models; Brain architecture; CEST; Correlation analysis; Creatine; exchange rate; Field strength; Foreign exchange rates; Glioma; In vitro methods and tests; Lysates; Mapping; Medical imaging; Metastases; Neuroimaging; omega plot; pH effects; Statistical analysis; Statistical tests; Therapeutic applications; Xenografts; Xenotransplantation; pH; omega plot; glioma; CEST; exchange rate
• ISSN: 1053-1807; 1522-2586; 1522-2586
• DOI: 10.1002/jmri.29241

Background Quantitative in‐situ pH mapping of gliomas is important for therapeutic interventions, given its significant association with tumor progression, invasion, and metastasis. Although chemical exchange saturation transfer (CEST) offers a noninvasive way for pH imaging based on the pH‐dependent exchange rate (ksw), the reliable quantification of ksw in glioma remains constrained due to technical challenges. Purpose To quantify the pH of gliomas by measuring the proton exchange rate through optimized omega plot analysis. Study Type Prospective. Phantoms/Animal Model/Subjects Creatine and murine brain lysates phantoms, six rats with glioma xenograft model, and three patients with World Health Organization grade 2–4 gliomas. Field Strength/Sequence 11.7 T, 7.0 T, CEST imaging, T2‐weighted (T2W) imaging, and T1‐mapping. Assessment Omega plot analysis, quasi‐steady‐state (QUASS) analysis, multi‐pool Lorentzian fitting, amine and amide concentration‐independent detection, pH enhanced method with the combination of amide and guanidyl (pHenh), and magnetization transfer ratio (MTR) were utilized for pH metric quantification. The clinical outcomes were determined through radiologic follow‐up and histopathological analysis. Statistical Tests Mann–Whitney U test was performed to compare glioma with normal tissue, and Pearson's correlation analysis was used to assess the relationship between ksw and other parameters. Results In vitro experiments reveal that the determined ksw at 2 ppm increases exponentially with pH (creatine phantoms: ksw = 106 + 0.147 × 10(pH‐4.198); lysates: ksw = 185.1 + 0.101 × 10(pH‐3.914)). Omega plot analysis exhibits a linear correlation between 1/MTRRex and 1/ω12 in the glioma xenografts (R2 > 0.98) and glioma patients (R2 > 0.99). The exchange rate in the rat glioma decreases compared to the contralateral normal tissue (349.46 ± 30.40 s−1 vs. 403.54 ± 51.01 s−1, P = 0.025), while keeping independence from changes in concentration (r = 0.5037, P = 0.095). Similar pattern was observed in human data. Data Conclusion Utilizing QUASS‐based, spillover‐, and MT‐corrected omega plot analysis for the measurement of exchange rates, offers a feasible method for quantifying pH within glioma. Level of Evidence NA Technical Efficacy Stage 1