The Genomes of Bovine Papillomaviruses Types 3 and 4 are Colinear

• Published in: Journal of general virology Vol. 64; no. 12; pp. 2771 - 2776
• Main Authors: Coggins, L. W, Hettich, I, Smith, K. T, Slater, A. A, Roe, F. A, Pfister, H, Campo, M. S
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.12.1983; Society for General Microbiology
• Subjects: Base Sequence; Biological and medical sciences; Bovine papillomavirus 1 - classification; Bovine papillomavirus 1 - genetics; Bovine papillomavirus 4; Deoxyribonuclease BamHI; Deoxyribonuclease EcoRI; DNA Restriction Enzymes; DNA, Viral; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genetics; Microbiology; Nucleic Acid Heteroduplexes; Nucleic Acid Hybridization; Papillomaviridae - genetics; Virology; Heteroduplex; Molecular hybridization; Genome organization; Bovine papillomavirus; Type; Transmission electron microscopy; DNA; Restriction map; Homology; Primary structure; Molecular cloning; Comparative study
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-64-12-2771

1 Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, U.K. 2 Institut für Virologie, Zentrum für Hygiene, Universität Freiburg, Hermann Herder Strasse 11, 7800 Freiburg, F.R.G. and 3 Institut für Klinische Virologie, der Universität Erlangen-Nürnberg, Loschgestrasse 7, 8520 Erlangen, F.R.G. The 7.2 kb genomic DNA of bovine papillomavirus type 3 (BPV-3) was molecularly cloned using its unique Eco RI site, and the 7.3 kb genome of BPV-4 was cloned using its single Bam HI site. The viral genomes were compared by liquid hybridization, Southern blot hybridization and heteroduplex mapping. Low stringency hybridization conditions revealed that the genomes are colinear but the sequences are extensively mismatched. The relative alignment of the restriction endonuclease maps of the two viral genomes has been determined. It was found that the genomes as linearized for cloning are out of phase by 1.7 kb, so that the single Eco RI site of BPV-3 appears to coincide with the BPV-4 Eco RI site at 0.22 map units. It is concluded that the genomes of BPV-3 and BPV-4, both of which cause true epithelial warts, share the same physical organization but exhibit sequence divergence. Keywords: BPV, homology, genome Received 28 June 1983; accepted 1 September 1983.