Bioinspired, Bioactive, and Bactericidal: Anodized Nanotextured Dental Implants

• Published in: Advanced functional materials Vol. 34; no. 30
• Main Authors: Chopra, Divya, Guo, Tianqi, Jayasree, Anjana, Gulati, Karan, Ivanovski, Sašo
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc 01.07.2024
• Subjects: Adhesion; anodization; Anodizing; antibacterial; Antiinfectives and antibacterials; bioactive; Biological activity; Control surfaces; Dental implants; Effectiveness; nanopillars; Orthopedics; Osteoblasts; Papillae; Substrates; Surface properties; Texturing; Titanium; Wettability
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.202314031

Bioinspired titanium implant nanotexturing achieved via advanced techniques such as lithography, hydrothermal and laser patterning enables modulation of cell functions toward enhanced bioactivity and antibacterial efficacy. However, such costly, multi‐step methods limit clinical translation and benchtop implant modification. For the first time, single‐step, cost‐effective, and translatable electrochemical anodization is reported to fabricate bioinspired nanopillar‐like textures on micro‐rough titanium. In‐depth surface characterization confirms the formation of novel nanostructures, namely nanoscale Spinules, Daggers, Papillae, Spikes, and Flames, exhibiting varied roughness and wettability. Next, in separate experiments, primary human osteoblasts and polymicrobial salivary biofilm are cultured on the implant substrates. Nanotextured surfaces show high protein adhesion and maintain the proliferation, adhesion, and spreading of osteoblasts. Interestingly, all nanotextures exhibit superior antibiofilm abilities compared to control surfaces. Bioactive and antibacterial implant surface nano‐texturing achieved via single‐step anodization has the potential for clinical translation as the next generation of orthopedic and dental implant surface modification. Nanotextured titanium implants combat poor integration and infection, the primary causes of dental implant failure. This pioneering study utilizes anodization to create diverse nanotopographies, including Spinules, Daggers, Papillae, Spikes, and Flames. These bioinspired nanotextures enhance surface roughness, promote human osteoblast functions and exhibit superior efficacy against oral salivary biofilm.