Identification of SSR markers associated with seed coat permeability and electrolyte leaching in soybean

• Published in: Physiology and molecular biology of plants Vol. 14; no. 3; pp. 173 - 177
• Main Authors: Singh, R. K., Raipuria, R. K., Bhatia, V. S., Rani, Anita, Pushpendra, Husain, S. M., Tara Satyavathi, C., Chauhan, G. S., Mohapatra, T.
• Format: Journal Article
• Language: English
• Published: India Springer-Verlag 01.07.2008
• Subjects: Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Cell Biology; Life Sciences; Plant Physiology; Plant Sciences; Research Article; QTL; Seed coat permeability; SSR; Soybean; Electrolyte leaching
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-008-0016-0

Seed coat permeability and electrolyte leaching are the important traits that have been negatively associated with seed longevity in soybean. The objective of this study was to use SSR markers to identify genomic regions significantly associated with QTLs controlling seed coat permeability and electrolyte leaching in a segregating F 2 population derived from a cross of Birsa soya-1 x JS 71-05. Parental polymorphism survey using 145 SSR markers identified 21 polymorphic ones, which were used to genotype 153 F 2 individuals. Four independent markers (Satt434, Satt538, Satt281 and Satt598) were significantly (P=0.05) associated with seed coat permeability. One of these markers (Satt 281) also showed significant association with electrolyte leaching that partly supported the observed positive correlation (r = 0.425) between the two traits. Markers for seed coat permeability individually explained 3.9% to 4.5% of the total phenotypic variation, while the marker linked with electrolyte leaching explained 5.6% of the total variation.