Transcriptome of miRNA during inhibition of Bombyx mori nuclear polyhedrosis virus by geldanamycin in BmN cells

• Published in: Archives of insect biochemistry and physiology Vol. 110; no. 1; pp. e21880 - n/a
• Main Authors: Zhao, Zhi‐meng, Yin, Hao‐tong, Shen, Man‐man, Zhang, Shao‐lun, Chen, Zi‐kang, Li, Tao, Zhang, Zhen‐dong, Zhao, Wei‐guo, Guo, Xi‐jie, Wu, Ping
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.05.2022
• Subjects: Animals; Apoptosis; Baculoviridae; Benzoquinones; Bioinformatics; BmNPV; Bombyx - metabolism; Bombyx mori; Cell proliferation; Endocytosis; Geldanamycin; HSP90 inhibitor; Hsp90 protein; Lactams, Macrocyclic; lncRNA–miRNA–mRNA network; microRNA; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Nucleopolyhedroviruses - physiology; Polymerase chain reaction; Proteasomes; Proteolysis; Ribonucleic acid; RNA; RNA, Messenger - metabolism; Sericulture; Silkworms; silkworm–pathogen interaction; Transcriptome; Transcriptomes; Ubiquitin; Viruses; microRNA; lncRNA-miRNA-mRNA network; BmNPV; HSP90 inhibitor; silkworm-pathogen interaction
• ISSN: 0739-4462; 1520-6327
• DOI: 10.1002/arch.21880

Bombyx mori nuclear polyhedrosis virus (BmNPV) is one of several viruses that cause great harm to the sericulture industry, and its pathogenic mechanism is still being explored. Geldanamycin (GA), a kind of HSP90 inhibitor, has been verified to suppress BmNPV proliferation. However, the molecular mechanism by which GA inhibits BmNPV is unclear. MicroRNAs (miRNAs) have been shown to play a key role in regulating virus proliferation and host‐pathogen interactions. In this study, BmN cells infected with BmNPV were treated by GA and DMSO for 72 h, respectively, then transcriptome analysis of miRNA was performed from the GA group and the control group. As a result, a total of 29 miRNAs were differentially expressed (DE), with 13 upregulated and 16 downregulated. Using bioinformatics analysis, it was found that the target genes of DEmiRNAs were involved in ubiquitin‐mediated proteolysis, phagosome, proteasome, endocytosis pathways, and so on. Six DEmiRNAs were verified by quantitative reverse‐transcription polymerase chain reaction. DElong noncoding RNA (DElncRNA)–DEmiRNA–messenger RNA (mRNA) regulatory networks involved in apoptosis and immune pathways were constructed in GA‐treated BmN cells, which included 12 DEmiRNA, 132 DElncRNA, and 69 mRNAs. This regulatory network enriched the functional role of miRNA in the BmNPV‐silkworm interactions and improved our understanding of the molecular mechanism of HSP90 inhibitors on BmNPV proliferation. Figure Identification of microRNAs (miRNAs) in Bombyx mori nuclear polyhedrosis virus (BmNPV)‐infected BmN cells in response to HSP90 inhibitor. (a) Identification of miRNAs in geldanamycin (GA)‐treatment and control BmN cells infected with BmNPV infection. GA is a kind of HSP90 inhibitor. G represents GA‐treatment group. C represents control group; (b) validation of different expression of miRNA by qRT‐PCR; (c) partial display of DEmiRNA–DElong noncoding RNA (DElncRNA) network in response to HSP90 inhibitor. Diamonds indicate miRNAs and circles indicate lncRNAs. Red represents upregulation and green represents downregulation.