A dried blood spot‐based method to measure levels of tartrate‐resistant acid phosphatase 5b (TRACP‐5b), a marker of bone resorption

• Published in: American journal of human biology Vol. 31; no. 3; pp. e23240 - n/a
• Main Authors: Eick, Geeta N., Devlin, Maureen J., Cepon‐Robins, Tara J., Kowal, Paul, Sugiyama, Lawrence S., Snodgrass, J. Josh
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.05.2019; Wiley Subscription Services, Inc
• Subjects: Acid phosphatase; Acid phosphatase (tartrate-resistant); Acid resistance; Adult; Aged; Aged, 80 and over; Assaying; Biocompatibility; Biomarkers; Biomarkers - blood; Biomedical materials; Blood; Bone growth; Bone resorption; Bone Resorption - blood; Bone turnover; Dried Blood Spot Testing - instrumentation; Dried Blood Spot Testing - methods; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Female; Humans; Levels; Male; Middle Aged; Osteogenesis; Phosphatase; Population studies; Reliability analysis; Stability analysis; Tartrate-Resistant Acid Phosphatase - blood; Young Adult
• ISSN: 1042-0533; 1520-6300
• DOI: 10.1002/ajhb.23240

Objectives A number of basic questions about bone biology have not been answered, including population differences in bone turnover. In part, this stems from the lack of validated minimally invasive biomarker techniques to measure bone formation and resorption in field‐based population‐level research. The present study addresses this gap by validating a fingerprick dried blood spot (fDBS) assay for tartrate‐resistant acid phosphatase 5b (TRACP‐5b), a well‐defined biomarker of bone resorption and osteoclast number. Methods We adapted a commercially available enzyme‐linked immunosorbent assay (ELISA) kit from MyBiosource for the quantitative determination of TRACP‐5b levels in serum and plasma for use with DBS. We used a rigorous process of assay modification and validation, including the use of a matched set of 189 adult plasma, fDBS, and venous DBS (vDBS) samples; parameters evaluated included precision, reliability, and analyte stability. Results Plasma and DBS TRACP‐5b concentrations showed a linear relationship. There were no systematic differences in TRACP‐5b levels in fDBS and vDBS, indicating no significant differences in TRACP‐5b distribution between capillary and venous blood. Parallelism and spike‐and‐recovery results indicated that matrix factors in DBS do not interfere with measurement of TRACP‐5b levels from DBS using the validated kit. Intra‐ and interassay CVs were 5.0% and 12.1%, respectively. DBS samples should preferably be stored frozen but controlled room temperature storage for up to a month may be acceptable. Conclusions This DBS‐based ELISA assay adds to the methodological toolkit available to human biologists and will facilitate research on bone turnover in population studies.