Histology and ultrastructure of the testis and vas deferens in Pseudochorthippus parallelus parallelus (Orthoptera, Acrididae)

• Published in: Microscopy research and technique Vol. 82; no. 9; pp. 1461 - 1470
• Main Authors: Polat, Irmak, Amutkan Mutlu, Damla, Ünal, Mustafa, Suludere, Zekiye
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.09.2019; Wiley Subscription Services, Inc
• Subjects: Acrididae; Animals; Follicles; Gametocytes; Germ cells; Grasshoppers - anatomy & histology; Grasshoppers - physiology; Grasshoppers - ultrastructure; Histology; light microscope; Male; male reproductive system; Maturation; meadow grasshopper; Meiosis; Microscopy; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Mitosis; Organs; Orthoptera; Pseudochorthippus parallelus; Reproductive organs; scanning electron microscope; Scanning electron microscopy; Species; Sperm; Spermatids; Spermatids - cytology; Spermatocytes; Spermatogenesis; Spermatozoa; Spermiogenesis; Testes; Testis - anatomy & histology; Testis - physiology; Testis - ultrastructure; transmission electron microscope; Turkey; Ultrastructure; Vas deferens; Vas Deferens - anatomy & histology; Vas Deferens - ultrastructure; Vasectomy; Turkey; light microscope; meadow grasshopper; scanning electron microscope; male reproductive system; transmission electron microscope
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.23300

Pseudochorthippus parallelus parallelus (Zetterstedt, 1821) (Orthoptera, Acrididae) is a widespread species in Europe, and also it is localized in some regions in Turkey such as Bursa, Eskişehir, Ankara, Bolu, Düzce, and Çankırı. The features of the reproductive organs such as the numbers and shapes of testes and follicles can be used as taxonomical characters. For this purpose, the ultrastructural and histological features of testis and vas deferens in P. parallelus parallelus were examined with using light microscope, scanning electron microscope, and transmission electron microscope. The mature P. parallelus parallelus has two conjugated testes produce spermatozoa. Each testis is composed of numerous testis follicles in which different stages of spermatogenesis and spermiogenesis develop. First, spermatocytes are formed by the mitosis division of the germ cells at the distal end of the follicles. Then, spermatocytes form spermatids by meiosis division in the middle region of the follicles. Finally, spermatids are differentiated to spermatozoa at the proximal region of the follicles. After maturation of the spermatozoa, sperm tails come together as the sperm bundles called as spermatodesm. Each follicle is connected to vas deferens via vas efferens to discharging spermatozoa. In spite of some differences, the testes and the vas deferens in P. parallelus parallelus are highly similar to the those of other species, especially Orthopteran species. Investigate histological and ultrastructural features of testis in Pseudochorthippus parallelus parallelus with light microscope, scanning and transmission electron microscopes. Differences and similarities of structural features of testis in P. parallelus parallelus with other species were revealed.