Single nucleotide polymorphisms, haplotype association and tumour expression of the vascular endothelial growth factor (VEGF) gene with lung carcinoma

• Published in: Gene Vol. 608; pp. 95 - 102
• Main Authors: Naykoo, Niyaz A, Dil-Afroze, Rasool, Roohi, Shah, Sonaullah, Ahangar, A.G, Bhat, Imtiyaz A, Qasim, Iqbal, Siddiqi, Mushtaq A, Shah, Zafar A
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.04.2017
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma - pathology; Adult; Aged; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Case-Control Studies; Expression; Female; Gene Expression Regulation, Neoplastic; Genetic Association Studies; Genetic Predisposition to Disease; Haplotype; Haplotypes; Humans; Lung; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Male; Middle Aged; Polymorphism, Single Nucleotide; SNP; Vascular Endothelial Growth Factor A - genetics; VEGF; RNA; Lung; mRNA; CIs; SE; UTR; SNP; RT-PCR; SNPs; cDNA; qPCR; AIC; Haplotype; UV; OR; SKIMS; VEGF; EM; FPRP; μl; CT; SCC; CRC; Expression; DNA; GAPDH; ARMS-PCR; PCR
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2017.01.007

VEGF contains several polymorphic sites known to influence its expression. We examined the possible association between+405(−634)C>G,+936C>T,−2578C>A and lung cancer in 199 Kashmiri patients and 401 healthy controls. VEGF+405CG,+936CT+TT and−2578CA genotypes were significantly associated with lung cancer risk compared to VEGF+405CC,+936CC and−2578AA+CC genotypes [OR=0.07 (0.04−0.13), P<0.0001, OR=0.36 (0.25−0.52), P<0.0001 and 0.08 (0.05−0.13), P<0.0001]. Haplotype analysis revealed that CGA and TGA haplotypes of VEGF gene conveys the risk for lung cancer [OR=0.18 (0.10−0.33), P<0.0001 and 0.07 (0.03−0.13), P<0.0001]. VEGF expression revealed non-significant association with the genotypes of the three SNPs. In conclusion, the SNPs examined appear to influence lung cancer susceptibility while as genotypes of the SNPs don’t appear to have significant association with VEGF mRNA expression in lung tumours. •VEGF 936 CT+TT and −2578 CA were significantly associated with lung cancer.•CCC haplotype of VEGF conveys the risk for lung cancer compared with the CGA.•VEGF expression revealed non-significant association with genotypes.