Estimation of a steroid released from the crude saponins of the starfish, Asterias vulgaris, by solvolysis: Seasonal and geographic abundance

Conditions were optimized for the quantitative extraction and isolation, by reversed-phase adsorption, of a saponin, from the common or purple starfish, Asterias vulgaris, and for the subsequent solvolytic release and quantitation of a steroid aglycone, 5α-pregn-9(11)-ene-3β,6α-diol-20-one. With the...

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Published inAnalytical biochemistry Vol. 81; no. 1; pp. 196 - 208
Main Authors Burns, B.G., Gilgan, M.W., Logan, V.H., Burnell, J., ApSimon, J.W.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.1977
Subjects
Animals
Chromatography, Ion Exchange - methods
Geography
Pregnenes - analysis
Pregnenes - metabolism
Saponins - isolation & purification
Saponins - metabolism
Seasons
Starfish - metabolism
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Summary:Conditions were optimized for the quantitative extraction and isolation, by reversed-phase adsorption, of a saponin, from the common or purple starfish, Asterias vulgaris, and for the subsequent solvolytic release and quantitation of a steroid aglycone, 5α-pregn-9(11)-ene-3β,6α-diol-20-one. With the method developed, the abundance of the aglycone was found to vary seasonally and was greatest from April to June at two locations on the Atlantic coast of Nova Scotia. When several locations were examined during October, the abundance of the aglycone was also found to vary geographically.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(77)90613-3