Cellulose/biopolymer/Fe3O4 hydrogel microbeads for dye and protein adsorption

• Published in: Cellulose (London) Vol. 27; no. 5; pp. 2757 - 2773
• Main Authors: Park, Saerom, Oh, Yujin, Yun, Jeongchel, Yoo, Eunjin, Jung, Dahun, Oh, Kyeong Keun, Lee, Sang Hyun
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.03.2020; Springer Nature B.V
• Subjects: Adsorption; Biocompatibility; Biodegradability; Bioorganic Chemistry; Biopolymers; Blending; Carrageenan; Cellulose; Ceramics; Chemistry; Chemistry and Materials Science; Chitosan; Composites; Controllability; Dyes; Glass; Hydrogels; Iron oxides; Lignin; Lipase; Lysozyme; Nanoparticles; Natural Materials; Organic Chemistry; Original Research; Pepsin; Physical Chemistry; Polymer Sciences; Protein adsorption; Serum albumin; Sol-gel processes; Surface properties; Sustainable Development; Microbeads; Dye; Adsorption; Protein; Cellulose; Biopolymer
• ISSN: 0969-0239; 1572-882X
• DOI: 10.1007/s10570-020-02974-5

Cellulose-based magnetic hydrogel microbeads were prepared through sol–gel transition using a 1-ethyl-3-methylimidazolium acetate-in-oil emulsion. Surface properties of the microbeads were altered by blending cellulose with chitosan, carrageenan, lignin, or starch. The adsorption capacity of the cellulose microbeads for crystal violet was 1.3 times higher after blending cellulose with carrageenan, while that for methyl orange was 2.0 times higher after blending cellulose with chitosan. As a model study, kinetics and isotherms for the adsorption of crystal violet on the cellulose/carrageenan microbeads were investigated to understand the effect of the biopolymer on the adsorption properties. Adsorption capacities of the cellulose microbeads for pepsin and bovine serum albumin were 1.6 and 1.2 times higher after blending cellulose with chitosan, respectively. The adsorption capacity of the cellulose/carrageenan microbeads for lysozyme was 1.2 times higher than that of the cellulose microbeads. The cellulose/alkali lignin and cellulose/starch magnetic microbeads were found to be efficient supports for immobilization of lipase. Specific activities of lipase immobilized on the cellulose/alkali lignin and cellulose/starch magnetic microbeads were 1.2- and 1.4-fold higher than that of free lipase, respectively. Under denaturing thermal conditions, the half-life of lipase immobilized on the cellulose/alkali lignin and cellulose/starch magnetic microbeads was 47- and 56-fold higher than that of free lipase, respectively. Thus, owing to their biocompatibility, biodegradability, and controllability, the cellulose/biopolymer/Fe 3 O 4 hydrogel microbeads may have many potential applications in biocatalytic, biomedical, and environmental fields.