A biorefinery based on brewer`s spent grains: Arabinoxylans recovery by microwave assisted pretreatment integrated with butanol production

[Display omitted] •Integration of AX recovery with butanol production to use BSG in a biorefinery.•AX production of 133 kg/t BSG by microwave assisted alkaline pretreatment.•AX with a degree of polymerization from 3 to 27 was observed.•9.9 g/L butanol after ABE fermentation of the microwave alkaline...

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Bibliographic Details
Published inIndustrial crops and products Vol. 158; p. 113044
Main Authors López-Linares, Juan C., Lucas, Susana, García-Cubero, M. Teresa, Jiménez, Juan José, Coca, Mónica
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.12.2020
Subjects
ABE fermentation
Biorefinery
Brewing industry waste
Microwave pretreatment
Xylooligosaccharides
ABE fermentation
Brewing industry waste
Biorefinery
Microwave pretreatment
Xylooligosaccharides
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Summary:[Display omitted] •Integration of AX recovery with butanol production to use BSG in a biorefinery.•AX production of 133 kg/t BSG by microwave assisted alkaline pretreatment.•AX with a degree of polymerization from 3 to 27 was observed.•9.9 g/L butanol after ABE fermentation of the microwave alkaline pretreated solid.•ABE fermentation yield of 37 kg/t BSG, of which 28 kg/t are butanol. This study proposes an integrated biorefinery of brewer’s spent grain (BSG) based on the application of a microwave pretreatment to obtain bioproducts, such as arabinoxylans (AX) and an advanced biofuel, such as biobutanol. A prehydrolysate with 17 g/L AX was obtained at 172 °C and 0.38 M NaOH, which were subsequently precipitated using an organosolvent process, recovering 133 kg AX/t BSG. The subsequent enzymatic hydrolysis with endo-xylanases significantly increased the concentration of oligomers with 2–6 units of xylose. The presence of oligosaccharides with a degree of polymerization (DP) from 3 to 27 was confirmed by MALDI-TOF MS. On the other hand, the pretreated solid residue obtained in the microwave assisted alkaline pretreatment was enzymatically hydrolyzed with cellulases and fermented by Clostridium beijerinckii, producing 9.9 g/L butanol (28 kg butanol/t BSG and 37 kg ABE/t BSG). Thus, the potential for the efficient use of BSG in an integrated biorefinery was demonstrated.
ISSN:0926-6690
1872-633X
DOI:10.1016/j.indcrop.2020.113044