The Mn-modified porphyrin metal-organic framework with enhanced oxidase-like activity for sensitively colorimetric detection of glutathione

• Published in: Biosensors & bioelectronics Vol. 213; p. 114446
• Main Authors: Lai, Xiang, Shen, Yue, Gao, Shoubei, Chen, Yajing, Cui, Yanshuai, Ning, Dongxue, Ji, Xianbing, Liu, Zhiwei, Wang, Longgang
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.10.2022
• Subjects: Biosensing Techniques - methods; Colorimetry; Colorimetry - methods; Detection; Glutathione; Glutathione - chemistry; Humans; Hydrogen Peroxide - chemistry; Limit of Detection; Manganese - chemistry; Metal-Organic Frameworks - chemistry; Oxidase-like activity; Oxidoreductases - chemistry; PCN-224; Porphyrins; Colorimetry; Detection; PCN-224; Oxidase-like activity; Glutathione
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2022.114446

The selective detection of glutathione (GSH) has been used as important colorimetric probe for human health. Herein, we used a facile method to synthesize manganese ions modified porphyrin metal-organic framework (PCN-224-Mn) with a size of 125.7 ± 14.2 nm and zeta potential of −3.9 ± 0.5 mV. We showed that PCN-224-Mn catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the absence of H2O2, resulting in a blue-colored oxidized TMB (oxTMB) that exhibits oxidase-like activity. Furthermore, a simple colorimetric detection method for GSH was developed based on the oxidase-like activity of PCN-224-Mn. This method shows wide linear detection range of 0.5–60 μM for GSH with a much lower detection limit of 0.233 μM. Finally, the recovery of colorimetric sensor of PCN-224-Mn suggests its great potential as a biosensor. As the catalytically active site, the manganese porphyrin unit plays a major role in the oxidase-like property and detection ability of PCN-224-Mn. Our data suggest that GSH detection method using PCN-224-Mn has great potential in multiple applications in the future. Scheme of GSH detection based on PCN-224-Mn with oxidase-like property. [Display omitted]