Antibody-coated protein A-bearing Staphylococcus aureus: a versatile and stable immune reagent

The human beta2-microglobulin antigen-antibody system was used as a model to illustrate the versatility of a microradioimmunoassay technique using protein A-bearing Staphylococcus aureus Cowan I strain (SACI) bacteria as a non-specific immunoadsorbent in place of a second antibody. Experimental cond...

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Bibliographic Details
Published inJournal of immunological methods Vol. 25; no. 3; p. 255
Main Authors Natali, P G, Pellegrino, M A, Walker, L, Ferrone, S, Reisfeld, R A
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1979
Subjects
Animals
Antibody Specificity
Antigen-Antibody Reactions
beta 2-Microglobulin - immunology
Binding Sites, Antibody
Binding, Competitive
Humans
Immune Sera - pharmacology
Rabbits
Radioimmunoassay
Staphylococcal Protein A - immunology
Staphylococcus aureus - immunology
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Summary:The human beta2-microglobulin antigen-antibody system was used as a model to illustrate the versatility of a microradioimmunoassay technique using protein A-bearing Staphylococcus aureus Cowan I strain (SACI) bacteria as a non-specific immunoadsorbent in place of a second antibody. Experimental conditions are described for a sensitive microassay which makes it possible to process large numbers of samples more rapidly and with minimum handling. Furthermore, SACI coated with specific antibodies by mixing with unfractionated antisera are a versatile reagent not only for radioimmunoassays but also for use as molecular probes to characterize cell surface antigens. Antibody-coated SACI could be lyophilized and proved extremely stable in storage thus providing a unique advantage for use in binding inhibition assays and as versatile reagent for clinical and investigative immunology.
ISSN:0022-1759
DOI:10.1016/0022-1759(79)90113-3